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PXD069863-1
PXD069863 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Aging-associated SATB1 deficiency remodels 3D genome architecture and transcriptional programs in naïve CD4⁺ T cells |
| Description | Precise three-dimensional (3D) genome organization is essential for regulating gene expression during development, yet its role in age-related transcriptional changes and physiological decline remains incompletely understood. Here, we show that aging reshapes chromatin architecture and gene regulation in murine naïve CD4⁺ T cells, a quiescent T cell population essential for adaptive immunity. Aged naïve CD4⁺ T cells exhibit intrinsic transcriptional reprogramming marked by increased expression of inflammatory and T cell activation-related genes, rendering them more prone to activation. Intriguingly, these changes are accompanied by pronounced alterations in 3D genome organization, including widespread weakening of TAD boundaries and extensive enhancer–promoter rewiring. Mechanistically, we demonstrate that these age-associated structural changes can be partly attributed to the diminished expression of chromatin organizer SATB1. SATB1 colocalizes with CTCF in young naïve T cells to spatially constrain the interaction of CTCF binding sites. Its decline with age extends the range of CTCF-mediated interactions without altering CTCF binding, leading to remodeling of chromatin architecture and activation of proinflammatory genes. Conditional SATB1 deletion in naïve T cells recapitulates the 3D genome and transcriptional changes observed in aging. Our findings reveal a critical role for SATB1 in maintaining 3D genome integrity in naïve T cells, and suggest that the dysregulation of genome architecture contributes to immune and organismal aging. |
| HostingRepository | iProX |
| AnnounceDate | 2025-10-23 |
| AnnouncementXML | Submission_2026-08-25_00:40:40.306.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Bao Wang |
| SpeciesList | scientific name: Mus musculus; NCBI TaxID: 10090; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Q Exactive HF-X |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2025-10-23 20:12:42 | ID requested | |
| ⏵ 1 | 2026-08-25 00:40:40 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: CTCF, IP-Ms |
Contact List
| Qian Bian | |
|---|---|
| contact affiliation | Shanghai Jiaotong University |
| contact email | qianbian@shsmu.edu.cn |
| lab head | |
| Bao Wang | |
| contact affiliation | Shanghai Jiaotong University |
| contact email | wangbao161@126.com |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
