PXD069844 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Proximity interaction network of Rho GTPases by BioID in human podocytes |
| Description | Rho GTPases play critical roles in regulating podocyte cytoskeleton and their alteration leads to foot process effacement. However, their signaling networks remain poorly understood. To gain better insights into Rho GTPases signaling in human podocyte, we implemented the high-throughput proximity-dependent biotinylation assay (BioID) using 9 baits of Rac1, Cdc42 and RhoA covering their various nucleotide loading states: Control wild-type Rho GTPases (RhoA WT, Rac1 WT, and Cdc42 WT), nucleotide-free (NF) mutants (RhoAG17A, Rac1G15A, and Cdc42G15A), constitutively active mutants (RhoAG14V, Rac1G12V, and Cdc42G12V). |
| HostingRepository | PRIDE |
| AnnounceDate | 2025-11-03 |
| AnnouncementXML | Submission_2025-11-03_10:15:48.489.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Sajida Ibrahim |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | monohydroxylated residue; iodoacetic acid derivatized residue; deamidated residue |
| Instrument | Orbitrap Fusion |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-10-23 14:15:06 | ID requested | |
| ⏵ 1 | 2025-11-03 10:15:48 | announced | |
Publication List
Keyword List
| submitter keyword: BioID, RhoA, Rac1, Rho GTPases, Cdc42, human podocytes |
Contact List
| Dr. Tomoko Takano |
|---|
| contact affiliation | Department of Medicine, Division of Experimental Medicine, McGill University, Montreal, QC, Canada |
| contact email | tomoko.takano@mcgill.ca |
| lab head | |
| Sajida Ibrahim |
|---|
| contact affiliation | Research Institute of McGill University Health Centre |
| contact email | sajida.ibrahim@mail.mcgill.ca |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/11/PXD069844 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD069844
- Label: PRIDE project
- Name: Proximity interaction network of Rho GTPases by BioID in human podocytes
