PXD069807 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Proteomic dataset of MECP2-deficient and wild-type human brain organoids under spaceflight and ground conditions |
| Description | This dataset presents deep proteomic profiles of human brain organoids cultured aboard the International Space Station (ISS) and on Earth. We analyzed MECP2-deficient (Q83X) and isogenic wild-type control (WT83) organoids exposed to spaceflight and ground conditions for 30 days. Using LC-MS/MS on an Orbitrap Astral mass spectrometer, we confidently identified 56,639 peptides mapping to approximately 6,000 protein groups. The Q83X line models Rett syndrome, carrying a nonsense mutation that produces a severely truncated MeCP2 protein lacking critical functional domains. This unique dataset enables investigation of how spaceflight environment and MECP2 deficiency interact to affect human neural development, offering applications for understanding astronaut health risks and neurodevelopmental disorder mechanisms. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-02-09 |
| AnnouncementXML | Submission_2026-02-09_16:00:02.056.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Paulo Carvalho |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Orbitrap Astral |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-10-22 19:25:57 | ID requested | |
| ⏵ 1 | 2026-02-09 16:00:02 | announced | |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: astrocytes, proteomics,brain organoids, L1 retrotransposition, MECP2, microgravity, retrotransposons, neurodegeneration, senescence |
Contact List
| ALysson Muotri |
|---|
| contact affiliation | Director Sanford Stem Cell Education and Integrated Space Stem Cell Orbital Research (ISSCOR) Center Director Gene Therapy Initiative |
| contact email | muotri@health.ucsd.edu |
| lab head | |
| Paulo Carvalho |
|---|
| contact affiliation | Fiocruz |
| contact email | paulo@pcarvalho.com |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/02/PXD069807 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD069807
- Label: PRIDE project
- Name: Proteomic dataset of MECP2-deficient and wild-type human brain organoids under spaceflight and ground conditions
