PXD069782-1

PXD069782 is an original dataset announced via ProteomeXchange.

Title	Comparative label-free proteomic analysis of multiple CHO cell lines under tunicamycin-induced ER stress and proteasome inhibition
Description	Chinese Hamster Ovary (CHO) cells are widely utilised in the biopharmaceutical industry to produce therapeutic proteins. Understanding the mechanisms of endoplasmic reticulum (ER) stress and its interplay with protein degradation pathways remains pivotal for improving production efficiency and product quality. Proteasome inhibitors such as MG132 offer a valuable tool for studying ER stress by disrupting protein degradation and promoting the accumulation of ubiquitinated proteins. In this study, we investigated the proteomic responses of CHO-K1 (non-producer), CHO DP-12 and NISTCHO (IgG producer) cell lines under ER stress induced by a combination of the proteasome inhibitor MG132 and the glycosylation inhibitor tunicamycin. The combined treatment of MG132 and tunicamycin intensified ER stress by preventing the degradation of ubiquitinated misfolded proteins, activating the unfolded protein response (UPR) more robustly than either agent alone. Key pathways related to protein folding, ER- associated degradation (ERAD), and the ubiquitin-proteasome system were significantly altered, particularly in the producer cell line NISTCHO. By modulating stress responses and protein degradation pathways, this work contributes to improving the yield and quality of biopharmaceuticals in industrial production settings.
HostingRepository	PRIDE
AnnounceDate	2026-08-17
AnnouncementXML	Submission_2026-08-16_17:25:27.612.xml
DigitalObjectIdentifier	https://doi.org/10.6019/PXD069782
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Michael Henry
SpeciesList	scientific name: Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus); NCBI TaxID: NEWT:10029;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2025-10-22 09:40:54	ID requested
⏵ 1	2026-08-16 17:25:28	announced

Sideri CK, Ryan D, Henry M, Efeoglu E, Meleady P, Proteasome Inhibition Amplifies Endoplasmic Reticulum (ER) Stress Responses: Comparative Proteomics of Chinese Hamster Ovary Cell Lines. Biomolecules, 16(2):(2026) [pubmed]

10.3390/biom16020277;

10.6019/PXD069782;

submitter keyword: Stress, Tunicamycin,CHO, LC-MS/MS

Dr. Paula Meleady
contact affiliation	School of Biotechnology, Dublin City University, Dublin, Ireland
contact email	paula.meleady@dcu.ie
lab head
Michael Henry
contact affiliation	NICB - DCU
contact email	michael.henry@dcu.ie
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD069782
     1. Label: PRIDE project
     2. Name: Comparative label-free proteomic analysis of multiple CHO cell lines under tunicamycin-induced ER stress and proteasome inhibition