⮝ Full datasets listing
PXD069771-1
PXD069771 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Rapid and Label-Free Structural Proteomics by One-Step Swift Trypsin LiP-MS |
| Description | We developed a rapid, surface-targeted limited proteolysis workflow, Swift Trypsin LiP-MS (STLiP-MS), to profile proteome-wide structural changes under near-native conditions. STLiP-MS uses a trypsin-immobilized spin column and centrifugation-controlled contact to achieve instantaneous, highly reproducible limited digestion, followed by reduction/alkylation and desalting. Proof-of-concept analyses were performed on HEK293T cell lysates (± phosphatase inhibitor PhosSTOP) and on a purified GPCR complex (A2A-BRIL with IgG). Peptides were separated on a 75-µm ID C18 column (50 °C) using an UltiMate 3000 RSLCnano and analyzed on an Orbitrap HF-X. Both DDA (top-50 HCD, 70-min gradient) and DIA (variable windows, 70-min gradient) runs were acquired. DIA data were processed with DIA-NN (library-free/in-silico; UniProt human, 2024-03), and DDA of purified samples with PEAKS Studio 12.5. |
| HostingRepository | jPOST |
| AnnounceDate | 2026-01-05 |
| AnnouncementXML | Submission_2026-01-04_16:18:57.429.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Yusei Okuda |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; scientific name: cellular organisms; NCBI TaxID: 131567; |
| ModificationList | S-carboxamidomethyl-L-cysteine; unknown modification; L-methionine sulfoxide |
| Instrument | Q Exactive HF-X |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2025-10-22 03:19:48 | ID requested | |
| ⏵ 1 | 2026-01-04 16:18:57 | announced | |
| 2 | 2026-01-19 19:15:34 | announced | 2026-01-20: Updated PubMed. |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: Lip-MS, One-step digestion, Structural proteomics, Protein–protein interactions |
Contact List
| Yusuke Kawashima | |
|---|---|
| lab head | |
| Yusei Okuda | |
| contact affiliation | Kitasato University |
| dataset submitter | |
Full Dataset Link List
| jPOST dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST004097/ |
