PXD069766-1

PXD069766 is an original dataset announced via ProteomeXchange.

Title	A MALAT1-encoded micropeptide acts as a potent enhancer of Influenza A Virus replication
Description	In this study, we investigated the regulation of lncRNA MALAT1 expression and its role during the influenza A virus (IAV) infection. We found that IAV infection robustly upregulated the expression of MALAT1 in vitro and in vivo. Furthermore, we demonstrated that MALAT1 expression was regulated via the NF-κB/IL-6/STAT3 pathway in host cells infected with IAV. Functional studies revealed that disruption of MALAT1 expression inhibited IAV replication, whereas overexpression of certain MALAT1 fragments enhanced the virus replication. Using ribosome profiling, mass spectrometry, and antibody validation, we identified a micropeptide (miPEP-52) encoded by an RNA fragment of MALAT1, which was endogenously expressed and upregulated by IAV infection. Moreover, we observed that miPEP-52 strongly enhanced the replication of IAV. Mutating the miPEP-52 start codon or deleting its coding sequence from the MALAT1 RNA fragment abolished these effects.
HostingRepository	iProX
AnnounceDate	2025-10-21
AnnouncementXML	Submission_2025-10-22_01:19:19.597.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Song Wang
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2025-10-22 01:19:03	ID requested
⏵ 1	2025-10-22 01:19:20	announced

Dataset with its publication pending

submitter keyword: Influenza A virus, lncRNA, MALAT1, micropeptide, innate immunity

Song Wang
contact affiliation	Fujian Agriculture and Forestry University
contact email	wscookie@163.com
lab head
Song Wang
contact affiliation	Fujian Agriculture and Forestry University
contact email	wscookie@163.com
dataset submitter

iProX dataset URI