PXD069760-1

PXD069760 is an original dataset announced via ProteomeXchange.

Title	Multi-Omics Insights into the Effects of Alkaline Mineral Ion Complex Supplementation on Colonic Health in High-Concentrate Diet-Fed Cattle
Description	High-concentrate diets commonly used in finishing beef cattle can impair colonic health by disrupting the intestinal barrier, promoting inflammation, oxidative stress, and electrolyte imbalances. This study aimed to evaluate the effects of alkaline mineral ion complex (AMC) supplementation on colonic health in cattle under high-concentrate feeding conditions. Results demonstrated that AMC supplementation significantly enhanced colonic barrier integrity by upregulating tight junction proteins and increasing goblet cell numbers, thereby improving mucosal defense. AMC also mitigated inflammatory cytokines (IL-1β, IL-6) and oxidative stress (reduced MDA and increased GSH-Px). Additionally, AMC supplementation restored electrolyte balance by enhancing Na⁺, K⁺, and Ca reabsorption, likely through upregulation of ion transporters and improvement of intestinal barrier function. The microbial ecosystem was also reshaped by AMC, with increased α-diversity and a reduction in pathogenic bacterial populations. Metabolomic and proteomic analyses revealed the downregulation of sphingolipid signaling pathway, particularly sphingosine, and the modulation of BCAA degradation pathways. These metabolic shifts suggest reduced inflammation and improved epithelial function. Collectively, AMC supplementation offers a promising dietary intervention to restore gut homeostasis, reduce inflammation, and enhance colonic health under high-concentrate feeding conditions.
HostingRepository	iProX
AnnounceDate	2025-10-22
AnnouncementXML	Submission_2025-10-22_00:58:24.269.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jia Li
SpeciesList	scientific name: Bos taurus; NCBI TaxID: 9913;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2025-10-22 00:58:11	ID requested
⏵ 1	2025-10-22 00:58:24	announced

Dataset with its publication pending

submitter keyword: Alkaline mineral ion complex (AMC)

High-concentrate diet

Colonic barrier function

Gut microbiota

Multi-omics analysis

Zhicai Zuo
contact affiliation	Sichuan Agricultural University
contact email	czyhzzc@126.com
lab head
Jia Li
contact affiliation	Sichuan Agricultural University
contact email	1011756736@qq.com
dataset submitter

iProX dataset URI