PXD069758-1
PXD069758 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Proteomics and Acetyl-proteomics reveal the antibacterial mechanism of berberine sulfate against methicillin-resistant Staphylococcus aureus |
| Description | The escalating prevalence of methicillin-resistant Staphylococcus aureus (MRSA) underscores the critical necessity for innovative antibacterial strategies. Berberine sulfate, a naturally occurring antimicrobial agent, demonstrates inhibitory effects against MRSA; however, its precise molecular targets and mechanisms of action remain inadequately defined. This study sought to systematically elucidate the regulatory mechanisms of berberine sulfate on MRSA, with particular emphasis on its influence on protein expression and post-translational modifications. Through quantitative proteomic analysis, 650 differentially expressed proteins were identified. Subsequent acetylome profiling revealed 38 differentially acetylated sites, among which berberine sulfate specifically induced K82 acetylation of the global regulator sarA. Functional analyses further revealed that the K82 mutant sarA exhibited impaired binding affinity to the agr promoter. These findings suggest a comprehensive dynamic response and potential molecular mechanism mediated by anti-MRSA effect of berberine sulfate. |
| HostingRepository | iProX |
| AnnounceDate | 2025-10-21 |
| AnnouncementXML | Submission_2025-10-22_00:54:24.378.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Junyu Xu |
| SpeciesList | scientific name: Staphylococcus aureus; NCBI TaxID: 1280; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Orbitrap Ascend |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2025-10-22 00:54:12 | ID requested | |
| ⏵ 1 | 2025-10-22 00:54:24 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: Proteomics, Acetylation, Staphylococcus aureus, berberine sulfate |
Contact List
| Junyu Xu | |
|---|---|
| contact affiliation | Chinese Academy of Sciences Shanghai Institute of Materia Medica |
| contact email | jyxu@simm.ac.cn |
| lab head | |
| Junyu Xu | |
| contact affiliation | Chinese Academy of Sciences Shanghai Institute of Materia Medica |
| contact email | jyxu@simm.ac.cn |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
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