PXD069749-1

PXD069749 is an original dataset announced via ProteomeXchange.

Title	Occupationally Relevant Vibrations and the Brain: Frequency-Dependent Proteomics Signatures in a Rat Model
Description	Occupational exposure to whole-body vibration (WBV), particularly in agricultural settings, has been linked to adverse cognitive and physiological outcomes. This study investigates the neurophysiological effects of WBV using a rat model exposed to sinusoidal vibrations at 4 Hz and 30 Hz, frequencies relevant to machinery operation. Forty-four Sprague-Dawley rats were randomly assigned to control (0 Hz), low-frequency (4 Hz), or high-frequency (30 Hz) vibration conditions. Following three days of exposure, brain tissues were harvested and analyzed using mass spectrometry-based proteomics to identify differentially expressed proteins. Proteomics analysis revealed distinct frequency-dependent alterations in brain protein expression. A total of 32 cognition-related proteins were differentially regulated at 4 Hz compared to 0 Hz, 29 at 30 Hz compared to 0 Hz, and 13 between 4 Hz and 30 Hz. Principal component analysis demonstrated clear separation among groups, indicating unique proteomics signatures for each vibration condition. Functional enrichment and protein-protein interaction analyses revealed that differentially expressed proteins were involved in synaptic plasticity, cytoskeletal organization, calcium regulation, and neurotransmitter release. Notably, 4 Hz exposure was associated with upregulation of proteins linked to calcium homeostasis and synaptic integrity, suggesting potential cognitive disruption. In contrast, 30 Hz exposure resulted in the upregulation of proteins associated with axonal guidance and neuroprotection, indicating possible adaptive or beneficial effects. Comparative analysis between 4 Hz and 30 Hz conditions highlighted proteins such as syntaxin-1A and glutamate receptor 2, which may serve as biomarkers for cognitive impairment or resilience. This study provides novel insights into the biological mechanisms underlying WBV-induced cognitive changes and highlights the importance of vibration frequency in determining neurophysiological outcomes. These findings lay the groundwork for future research integrating proteomics with behavioral assessments to better understand the impact of WBV on brain function in both animals and humans.
HostingRepository	PRIDE
AnnounceDate	2026-06-27
AnnouncementXML	Submission_2026-06-27_14:55:44.926.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Daniel Chao
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: NEWT:10116;
ModificationList	carbamoylated residue; 2-pyrrolidone-5-carboxylic acid (Gln); phosphorylated residue; acetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	6550 iFunnel Q-TOF LC/MS

Revision	Datetime	Status	ChangeLog Entry
0	2025-10-21 18:12:43	ID requested
⏵ 1	2026-06-27 14:55:45	announced

Dataset with its publication pending

submitter keyword: Rat, Brain, LC-MS/MS

Dr. George S. Katselis
contact affiliation	Department of Medicine (CCRAH), College of Medicine, University of Saskatchewan
contact email	george.katselis@usask.ca
lab head
Daniel Chao
contact affiliation	Health Sciences, College of Medicine, University of Saskatchewan
contact email	djc959@usask.ca
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD069749
     1. Label: PRIDE project
     2. Name: Occupationally Relevant Vibrations and the Brain: Frequency-Dependent Proteomics Signatures in a Rat Model