PXD069738-1

PXD069738 is an original dataset announced via ProteomeXchange.

Title	Nanopriming with Spirulina carbon dots boosts translation, metabolism, and ion homeostasis in rice seedlings
Description	Biogenic carbon dots (CDs) are emerging plant biostimulants, yet their effects during early crop establishment remain underexplored. Here, we synthesize and characterize Spirulina-derived CDs and test their performance as seed nanopriming agents in rice. The CDs showed nanoscale dimensions and abundant surface functionalities, with Raman/FTIR features consistent with graphitic-amorphous carbon and a highly negative ζ-potential, indicative of stable dispersions. Near-infrared spectroscopy with PCA resolved time-dependent metabolic changes during imbibition and identified 8-12 h as an optimal priming window. At 0.2 mg mL-1 for 12 h, Spirulina nanopriming increased seed germination rate (25%), germination speed index (17%), vigor index I (22%), and root length (37%) relative to hydropriming. Additionally, nanoprimed seedlings accumulated more starch (24%) and total carbohydrates (8%), and exhibited higher total phenolics (20%) than hydroprimed seedlings, without evidence of oxidative imbalance. Proteomic profiling revealed coordinated reprogramming: Spirulina nanopriming selectively boosted pathways for protein synthesis, energy production, and ion/nutrient homeostasis, while pathways for reserve breakdown and constitutive defense declined, indicating a shift from stress-biased to growth-oriented metabolism. Collectively, Spirulina-derived CDs act as effective nanobiostimulants that couple early metabolic activation with nutrient mobilization, offering a practical route to strengthen rice seedling establishment.
HostingRepository	PRIDE
AnnounceDate	2026-08-17
AnnouncementXML	Submission_2026-08-16_17:36:39.293.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Vanildo Silveira
SpeciesList	scientific name: Oryza sativa (Rice); NCBI TaxID: NEWT:4530;
ModificationList	No PTMs are included in the dataset
Instrument	SYNAPT G2-Si

Revision	Datetime	Status	ChangeLog Entry
0	2025-10-21 13:03:08	ID requested
⏵ 1	2026-08-16 17:36:40	announced

Minello LVP, Aguzzoli C, Nunes A, Oliveira ER, Maraschin M, da Paschoa RP, Silveira V, Sperotto RA, L.) Germination, Crop Establishment, and Seedling Metabolic Performance. Plants (Basel), 15(5):(2026) [pubmed]

10.3390/plants15050770;

submitter keyword: Oryza sativa

Spirulina

Nanopriming

Carbon dots., microalgae,Biostimulant

Raul Antonio Sperotto
contact affiliation	Universidade Federal de Pelotas, Instituto de Biologia. Campus Capão do Leão - Prédio 14, Sala 304 Campus Universitário 96160000 - Capão do Leão, RS - Brasil Telefone: (51) 37147000
contact email	raulsperotto@gmail.com
lab head
Vanildo Silveira
contact affiliation	UNIVERSIDADE ESTADUAL DO NORTE FLUMINENSE
contact email	vanildo@uenf.br
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/08/PXD069738

PRIDE project URI

• PRIDE
  1. PXD069738
     1. Label: PRIDE project
     2. Name: Nanopriming with Spirulina carbon dots boosts translation, metabolism, and ion homeostasis in rice seedlings