PXD069732 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Proteograph™-Based Proteome and Sphingolipidome Analysis for Serological Biomarker Discovery in Long-Duration Space Mission |
| Description | Introduction: Exposure to microgravity for 180 days leads to significant muscle mass and strength loss, which current countermeasures only partially mitigate. This study aimed to identify novel serological biomarkers to monitor muscle tissue adaptations during and after spaceflight. Serum samples from eight astronauts (5 men and 3 women) were collected pre-flight, during flight (IF1, IF2), and post-flight (three days and one month after landing). Methods: Samples were analyzed using the Proteograph™ XT kit, enabling the detection of protein-level differences before and after flight. Mass spectrometry data were acquired in Data-Independent Acquisition (DIA) mode and analyzed with Perseus software using paired Student’s t-tests, with a false discovery rate (FDR) < 0.05. In addition, sphingolipids were analyzed by targeted mass spectrometry. Results: Among 1,718 detected proteins, 153 showed significant changes, with 11 displaying particularly marked alterations (e.g., PLIN1, PLIN3, and COL11A2), which were validated by immunoblotting. Functional pathway analysis identified ten pathways related to muscle function and cytoskeletal organization, and one associated with brain function. Moreover, levels of ceramides and sphingomyelins revealed a decrease in total ceramide in the first in-flight sample, followed by a rebound in subsequent samples, accompanied by changes in enzyme levels from muscle extracts. Conclusion: These findings provide new insights into physiological adaptations to microgravity and identify potential biomarkers for monitoring muscle health during long-duration space mission. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-05-13 |
| AnnouncementXML | Submission_2026-05-13_10:19:17.691.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Chiara guerrera |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
| Instrument | timsTOF HT |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-10-21 11:08:42 | ID requested | |
| ⏵ 1 | 2026-05-13 10:19:18 | announced | |
Publication List
Keyword List
| submitter keyword: glucosylceramide, Serological biomarkers, Spaceflight serum biomarkers, Serum proteomics, Sphingolipidomics,Proteograph, Long-duration space mission, HDL-binding protein (HDLBP) |
Contact List
| Ida Chiara Guerrera |
|---|
| contact affiliation | Proteomics Platform Necker, Université Paris Cité - Structure Fédérative de Recherche Necker, INSERM US24/CNRS UAR3633, Paris 75015, France. |
| contact email | chiara.guerrera@inserm.fr |
| lab head | |
| Chiara guerrera |
|---|
| contact affiliation | Necker proteomics, INSERM |
| contact email | chiara.guerrera@inserm.fr |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD069732 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD069732
- Label: PRIDE project
- Name: Proteograph™-Based Proteome and Sphingolipidome Analysis for Serological Biomarker Discovery in Long-Duration Space Mission
