PXD069723 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Colonic smooth-muscle BIN1 and ALDH1B1 loss triggers mitochondrial dysfunction and fibrosis in slow-transit constipation |
| Description | Slow-transit constipation (STC) is a debilitating disorder of colonic motility with poorly defined molecular underpinnings. While abnormalities in the enteric nervous system and interstitial cells of Cajal have been extensively studied, smooth-muscle–intrinsic mechanisms remain largely unexplored. Here, we combine spatially resolved proteomics of human colon with functional perturbations in primary human colonic smooth muscle cells (HcoSMCs) to uncover two regulators of motility: Bridging Integrator 1 (BIN1) and Aldehyde Dehydrogenase 1B1 (ALDH1B1). Both proteins were downregulated in colonic smooth muscle of STC patients, localized to fibrotic regions. Lentiviral knockdown of BIN1 or ALDH1B1 in HcoSMCs impaired ATP-evoked Ca²⁺ responses and contraction, disrupted mitochondrial architecture, and elevated reactive oxygen species. Mechanistically, BIN1 loss coincided with mitochondrial apoptotic signaling and extracellular matrix deposition, whereas ALDH1B1 loss induced mitophagy and NF-κB–driven inflammatory programs. Transcriptomic analyses further revealed convergence on profibrotic pathways, consistent with collagen accumulation observed in patient tissues. Together, our findings delineate a smooth-muscle–centric framework of STC pathogenesis and nominate BIN1 and ALDH1B1 as candidate regulators of intestinal motility and potential entry points for therapeutic intervention. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-09-07 |
| AnnouncementXML | Submission_2026-09-06_16:39:47.120.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Jianbo Liu |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Orbitrap Astral |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-10-21 05:34:30 | ID requested | |
| ⏵ 1 | 2026-09-06 16:39:48 | announced | |
Publication List
| 10.1002/advs.202523688; |
| Liu J, Zhang H, Qiao W, Liu R, Sun J, Li X, Li Q, Zhao D, Chen D, Li J, Dong S, BIN1 and ALDH1B1 Deficiency in Colonic Smooth Muscle Drives Mitochondrial Dysfunction and Fibrosis in Slow-Transit Constipation. Adv Sci (Weinh), 13(50):e23688(2026) [pubmed] |
Keyword List
| submitter keyword: Mitochondrial function |
| Fibrosis |
| Intestinal motility |
Contact List
| Jianbo liu |
|---|
| contact affiliation | Department of Gastrointestinal Surgery, Shandong Provincial Third Hospital, Shandong University, Jinan, China |
| contact email | 1339065544@qq.com |
| lab head | |
| Jianbo Liu |
|---|
| contact affiliation | Department of Gastrointestinal Surgery, Shandong Provincial Third Hospital, Shandong University, Jinan, China |
| contact email | 1339065544@qq.com |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD069723
- Label: PRIDE project
- Name: Colonic smooth-muscle BIN1 and ALDH1B1 loss triggers mitochondrial dysfunction and fibrosis in slow-transit constipation
