PXD069720-1

PXD069720 is an original dataset announced via ProteomeXchange.

Title	Substrate Characterization of Mycobacterial Kinase PknG
Description	In this study, we utilized a multi-omic approach, encompassing proteomics, phosphoproteomics, and interactomics, to examine the influence of mycobacterial PknG-mediated regulation on host cellular processes. Our findings suggest that host lysyl-tRNA synthetase (KARS) is a potential substrate of PknG within host cells and may modulate immune response injury by affecting the phosphorylation levels of the MAPKs/NF-κB signaling pathway. Furthermore, our research demonstrates that mycobacterial PknG specifically catalyzes the phosphorylation of KARS at the T592/T595 sites, which can suppress TNF-α secretion from immune cells. Our study revealed that the PknG-KARS phosphorylation signaling axis influences the reduction of inflammatory factors in macrophages.
HostingRepository	iProX
AnnounceDate	2025-10-21
AnnouncementXML	Submission_2025-10-21_02:28:31.633.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Junyu Xu
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2025-10-21 02:28:03	ID requested
⏵ 1	2025-10-21 02:28:32	announced

Dataset with its publication pending

submitter keyword: Serine/threonine-protein kinase PknG, Lysine--tRNA ligase (KARS), Phosphorylation of KARS T592/T595

Junyu Xu
contact affiliation	Chinese Academy of Sciences Shanghai Institute of Materia Medica
contact email	jyxu@simm.ac.cn
lab head
Junyu Xu
contact affiliation	Chinese Academy of Sciences Shanghai Institute of Materia Medica
contact email	jyxu@simm.ac.cn
dataset submitter

iProX dataset URI