PXD069714-1

PXD069714 is an original dataset announced via ProteomeXchange.

Title	Proteomic Antibacterial Characterization of Flavonoid Xanthohumol and probiotic Clostridium butyricum on Pathogenic Clostridioides difficile
Description	In this study, we evaluated the antimicrobial properties of XN and C. butyricum culture supernatant (CBs) against C. difficile, focusing on their ability to reduce C. difficile adhesion, inhibit growth, and restrict toxin production. Using a dextran sulfate sodium (DSS)-induced Caco-2 intestinal epithelial model, we demonstrated that XN alleviates C. difficile-induced epithelial barrier disruption and inhibits the activation of related inflammatory signaling pathways. Additionally, our research indicated that both XN and C. butyricum effectively prevent CDI-induced cellular damage and led to significant proteomic regulation in this pathogen. We then mapped the global acetylation landscape of C. difficile proteins response to these two conditions, revealing that lysine acetylation regulates pathogenicity by modulating glycolysis pathways and the activity of metabolic enzymes. These findings suggest that XN and CBs may represent promising therapeutic options for CDI, which serving as a valuable resource for investigating lysine acetylation in C. difficile metabolism and virulence.
HostingRepository	iProX
AnnounceDate	2025-10-21
AnnouncementXML	Submission_2025-10-21_01:30:03.585.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Junyu Xu
SpeciesList	scientific name: Clostridioides difficile ATCC 9689 = DSM 1296; NCBI TaxID: 1121308; scientific name: Clostridioides difficile 70-100-2010; NCBI TaxID: 1002369;
ModificationList	acetylated residue
Instrument	Orbitrap Ascend

Revision	Datetime	Status	ChangeLog Entry
0	2025-10-21 01:29:49	ID requested
⏵ 1	2025-10-21 01:30:04	announced

Dataset with its publication pending

submitter keyword: Clostridioides difficile, Xanthohumol, Clostridium butyricum, post-translational modification, glycolysis

Junyu Xu
contact affiliation	Chinese Academy of Sciences Shanghai Institute of Materia Medica
contact email	jyxu@simm.ac.cn
lab head
Junyu Xu
contact affiliation	Chinese Academy of Sciences Shanghai Institute of Materia Medica
contact email	jyxu@simm.ac.cn
dataset submitter

iProX dataset URI