PXD069689-1
PXD069689 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Orthogonal Ionic Liquid-based Extraction Strategy Enables Amyloid-Specific Profiling of the Aggregate Proteome |
| Description | Amyloid deposits are key pathological features of neurodegenerative diseases, yet their structural heterogeneity coupled with a stable β-sheet fibrillar architecture makes them extremely insoluble and difficult to isolate. Through systematic screening and molecular dynamics simulations, we identified the ionic liquid TMGBF4, which disrupts β-sheet hydrogen bonds to specifically and preferentially solubilize amyloid aggregates. By harnessing C12ImCl-mediated hydrophobic interactions, we developed an orthogonal ionic-liquid extraction strategy (Orth-iEA) for highly selective amyloid fibril isolation. Applied to an Alzheimer’s disease mouse model, Orth-iEA enabled in-depth amyloid proteome profiling, enhanced detection of low-abundance proteins, and revealed mitochondrial involvement in amyloid pathology. This methodology delivers a versatile chemical strategy advances amyloid research beyond compositional profiling, enabling high-fidelity isolation and deep proteomic analysis of aggregation-driven pathology. |
| HostingRepository | iProX |
| AnnounceDate | 2025-10-17 |
| AnnouncementXML | Submission_2025-10-20_17:55:59.690.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Shiying Zheng |
| SpeciesList | scientific name: Mus musculus; NCBI TaxID: 10090; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Orbitrap Exploris 480 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2025-10-20 17:55:47 | ID requested | |
| ⏵ 1 | 2025-10-20 17:56:00 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: Proteome data, Mass spectrometry data |
Contact List
| Lihua Zhang | |
|---|---|
| contact affiliation | Dalian Institute of Chemical Physics, Chinese Academy of Sciences |
| contact email | lihuazhang@dicp.ac.cn |
| lab head | |
| Shiying Zheng | |
| contact affiliation | Dalian Institute of Chemical Physics, Chinese Academy of Sciences |
| contact email | zhengshiying@dicp.ac.cn |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
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