PXD069623-1

PXD069623 is an original dataset announced via ProteomeXchange.

Title	Activation of NF-κB Signaling in Primary Macrophages by Plasma Derived Small Extracellular Vesicles from Head and Neck Cancer Patients
Description	Head and neck squamous cell carcinomas (HNSCC) are highly immunosuppressive, and tumor-associated macrophages comprise a major part in HNSCC tumor microenvironments. Here, we investigate the effects induced by small extracellular vesicles (sEVs) of HNSCC patients on primary macrophages. sEVs were isolated from plasma by size-exclusion chromatography. Internalization of PKH26-labeled sEVs was shown and mass spectrometry analysis was performed on HNSCC sEVs and sEV-treated macrophages. NF-κB activation was investigated by western blot, p65 translocation assay and proteome profiler. Downstream, mRNA and protein levels of cytokines and chemotaxis of T cells were investigated. Results from proteomic analysis revealed time-dependent modulation of the NF-κB signaling pathway, which was confirmed by other methods and caused altered downstream cytokine expression in macrophages. sEVs from HNSCC patients positive for human papilloma virus showed increased uptake of sEV and NF-κB activation. In conclusion, HNSCC sEVs significantly influenced macrophage proteome and NF-κB signaling, providing a potential target specifically targeting HPV(+), but not HPV(-) HNSCC.
HostingRepository	PRIDE
AnnounceDate	2026-09-07
AnnouncementXML	Submission_2026-09-06_17:22:21.688.xml
DigitalObjectIdentifier	https://doi.org/10.6019/PXD069623
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Monika Pietrowska
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2025-10-18 16:53:34	ID requested
⏵ 1	2026-09-06 17:22:22	announced

10.6019/PXD069623;

Huber D, Strachwitz FV, Hofmann L, Pietrowska M, Gawin M, Chiang DM, Guder C, Lotfi R, Kishida S, Pfaffl MW, Wollenberg B, Hoffmann TK, Brunner C, Theodoraki MN, B Signaling in Macrophages Depending on the HPV Status. Cancers (Basel), 18(14):(2026) [pubmed]

10.3390/cancers18142219;

submitter keyword: cancer, Human Papilloma Virus,Head and Neck Squamous Cell Carcinoma, small extracellular vesicles, macrophages, NF-κB, HNSCC

Monika Pietrowska
contact affiliation	Maria Sklodowska-Curie National Research Institute of Oncology, Gliwice Branch Gliwice, Poland
contact email	monika.pietrowska@gliwice.nio.gov.pl
lab head
Monika Pietrowska
contact affiliation	Maria Sklodowska-Curie National Research Institute of Oncology
contact email	monika.pietrowska@io.gliwice.pl
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD069623
     1. Label: PRIDE project
     2. Name: Activation of NF-κB Signaling in Primary Macrophages by Plasma Derived Small Extracellular Vesicles from Head and Neck Cancer Patients