PXD069607 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | High-Throughput Proteomics Sample Preparation Using 96-Channel Pipettor and Magnetic Pin Device |
| Description | The advent of high-throughput proteomics has created an urgent need for parallel increases in sample processing productivity. However, workflows—particularly those involving PTM profiling—remain highly complex and consist of multiple steps that demand exceptional reproducibility. Many laboratories are transitioning to liquid-handling systems to automate parts of the workflow, but the transition from manual pipetting to full automation of phosphoproteomics can be technically demanding and cost-prohibitive. Here, we describe a practical intermediate solution: the use of a manually operated 96-channel pipettor (Platemaster P220) in combination with a 96-well magnetic pin device (MagPin, VP Scientific). Using these devices, we demonstrate highly efficient and reproducible execution of a complete phosphoproteomics workflow in 96-well format, involving PAC digestion, desalting, phosphoenrichment and second round of desalting within just two days, with low operator workload, minimal sample preparation variability and virtually no risk of error. As part of the workflow, we also present a methodology for producing affordable, highly reproducible Oasis HLB 96-well SPE plates by directly packing HLB sorbent material into tapered filter plates. We comprehensively characterise these custom Oasis HLB plates with respect to loading capacity, acetonitrile percentage required for peptide elution, lipid removal efficiency, and suitability for high-pH fractionation. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-02-18 |
| AnnouncementXML | Submission_2026-02-17_18:54:04.498.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Pavel Shliaha |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: NEWT:4932; scientific name: Escherichia coli; NCBI TaxID: NEWT:562; |
| ModificationList | phosphorylated residue |
| Instrument | Q Exactive HF |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-10-17 16:45:27 | ID requested | |
| ⏵ 1 | 2026-02-17 18:54:05 | announced | |
Publication List
| 10.1021/acs.jproteome.5c01020; |
| Roumelioti G, Montoya A, Fisher GLM, Navarro EP, Woods A, Bennett J, Navaratnam N, Gonzalez-Carvajal O, Birch J, Pyman E, Yu S, Gruevska A, Vuillemenot LA, Lushchak O, Hall Z, Barr AR, Speck C, Vernia S, Scott WR, Gil J, Aragon L, Fets L, Carling D, Shliaha PV, High-Throughput Proteomics Sample Preparation Using a 96-Channel Pipettor and Magnetic Pin Device. J Proteome Res, 25(3):1647-1661(2026) [pubmed] |
Keyword List
| submitter keyword: Oasis HLB,Proteomics, SP3, phosphoproteomics, PAC, high-throughput samples preparation |
Contact List
| Pavel Shliaha |
|---|
| contact affiliation | Head of Proteomics at MRC Laboratory of Medical Sciences |
| contact email | pavel.shliaha@gmail.com |
| lab head | |
| Pavel Shliaha |
|---|
| contact affiliation | Imperial College London |
| contact email | pavel.shliaha@gmail.com |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/02/PXD069607 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD069607
- Label: PRIDE project
- Name: High-Throughput Proteomics Sample Preparation Using 96-Channel Pipettor and Magnetic Pin Device
