PXD069590-1

PXD069590 is an original dataset announced via ProteomeXchange.

Title	Decreased Fc fucosylation aligns with enhanced RSV-specific antibody functionality in adults compared to young children
Description	Respiratory syncytial virus (RSV) is a major cause of severe respiratory tract infections in infants and older adults. While vaccines have recently been approved for older adults and pregnant women, vaccine development for infants remains challenging due to the risk of vaccine-enhanced disease. Understanding immunological differences between target groups is essential for improving vaccination strategies, but no clear correlates of protection have been identified yet. Antibody Fc-mediated effector functions may play an important role. In a cohort of 24-month old children and adults (n=46 per group), we assessed RSV-specific IgG/IgA levels, IgG subclasses, avidity, Fc glycosylation, and neutralization, antibody-dependent NK cell activation (ADNKA), cellular phagocytosis (ADCP), and complement deposition (ADCD). Compared to children, antibodies from adults showed enhanced functionality. Furthermore, RSV-specific antibodies displayed differences in avidity and glycosylation patterns between the two groups, which might relate to differences in the number of previous exposures. Importantly, our data strongly suggest that IgG Fc afucosylation drives enhanced ADNKA capacity of RSV-specific antibodies. Our data provide a detailed overview of similarities and differences between the RSV-specific antibodies in children and adults. This information will support the ongoing quest for correlates of protection and the design of future vaccination strategies in different target populations.
HostingRepository	PRIDE
AnnounceDate	2025-11-05
AnnouncementXML	Submission_2025-11-05_03:22:57.090.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Wenjun Wang
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	complex glycosylation
Instrument	impact HD; maXis

Revision	Datetime	Status	ChangeLog Entry
0	2025-10-17 08:18:12	ID requested
⏵ 1	2025-11-05 03:22:57	announced

Dataset with its publication pending

submitter keyword: Fc-glycosylation, antibody maturation, respiratory virus, Fc-functionality

Wenjun Wang
contact affiliation	Center for Proteomics and Metabolomics, Leiden University Medical Center Leiden, the Netherlands
contact email	w.wang@lumc.nl
lab head
Wenjun Wang
contact affiliation	Leiden University Medical Center
contact email	w.wang@lumc.nl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/11/PXD069590

PRIDE project URI

• PRIDE
  1. PXD069590
     1. Label: PRIDE project
     2. Name: Decreased Fc fucosylation aligns with enhanced RSV-specific antibody functionality in adults compared to young children