PXD069579-1

PXD069579 is an original dataset announced via ProteomeXchange.

Title	Proteomic Profiling of an ALS Drosophila Model Reveals the Impact of Scaffold Degrader P3 on Head Tissue
Description	This project presents a quantitative proteomic analysis of Drosophila melanogaster head tissue expressing human FUS-P525L, using data-independent acquisition (DIA) mass spectrometry. To enable identification of the human transgene, mass spectrometry data were analyzed against a custom database containing the Drosophila melanogaster proteome (UniProt UP000000803) and the human FUS sequence. Head samples were processed through protein extraction, acetone precipitation, and in-solution tryptic digestion. LC-MS/MS data were acquired on a Thermo Scientific instrument and analyzed with the DIA-NN software (v1.8.1), with FDR controlled at 1%. The dataset enables the investigation of protein expression changes, including the transgenic human FUS, under P3 treatment.
HostingRepository	iProX
AnnounceDate	2025-10-17
AnnouncementXML	Submission_2025-10-17_00:34:27.209.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	YingChen Zhou
SpeciesList	scientific name: Drosophila melanogaster; NCBI TaxID: 7227;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ

Revision	Datetime	Status	ChangeLog Entry
0	2025-10-17 00:34:14	ID requested
⏵ 1	2025-10-17 00:34:27	announced

Dataset with its publication pending

submitter keyword: Stress Granule, Drosophila melanogaster, FUS

JiaHeng Tan
contact affiliation	Sun Yat-sen University
contact email	tanjiah@mail.sysu.edu.cn
lab head
YingChen Zhou
contact affiliation	Sun Yat-sen University
contact email	zhouych35@mail2.sysu.edu.cn
dataset submitter

iProX dataset URI