PXD069552-1

PXD069552 is an original dataset announced via ProteomeXchange.

Title	Proteomic profiling of Ruegeria in response to coral tissue extract
Description	Probiotic applications show great potential for coral restoration, though conventional probiotics may form only transient associations with hosts. A proof-of-concept study revealed that Ruegeria MC10, an evolutionarily endosymbiont lineage, could persistently colonize coral tissue and enhance host health throughout an 8-month field experiment. To elucidate the molecular mechanisms underlying successful symbiosis, we conducted a comparative proteomic analysis of three Ruegeria strains – the MC10 representative MC10-B4 and two coral-derived counterpart, MC0-A5 and MC15-BG7 - in response to metabolites from the coral Acropora pruinosa. Following 4-hour exposures to coral tissue extracts or autoclaved 0.22 μm-filtered seawater (AFSW) controls at 28°C during exponential phase, we performed comprehensive protein characterization by LC-MS/MS. Differential protein expression profiles revealed strain-specific adaptations.
HostingRepository	PRIDE
AnnounceDate	2026-03-20
AnnouncementXML	Submission_2026-03-20_07:29:44.116.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Mei Xie
SpeciesList	scientific name: Ruegeria sp. JZ08IS16; NCBI TaxID: NEWT:1293091;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2025-10-16 14:14:44	ID requested
⏵ 1	2026-03-20 07:29:44	announced

Dataset with its publication pending

submitter keyword: Ruegeria, coral-associated bacteria, comparative proteomics, coral tissue extract

Haiwei Luo
contact affiliation	Director, Simon F.S. Li Marine Science Laboratory, CUHK, Hong Kong Associate Professor, School of Life Sciences, CUHK, Hong Kong
contact email	haiweiluo@cuhk.edu.hk
lab head
Mei Xie
contact affiliation	The Chinese University of Hong Kong
contact email	xiemei95@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/03/PXD069552

PRIDE project URI

• PRIDE
  1. PXD069552
     1. Label: PRIDE project
     2. Name: Proteomic profiling of Ruegeria in response to coral tissue extract