PXD069512 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Signal Attrition in Whole Cell Crosslinking Mass Spectrometry |
| Description | We investigated points in the typical sample workflow that could contribute to the perceived low cross-linking yields. We demonstrate, through a 2-step cross-linking strategy called Click-Linking, that the chemical reaction is not the main contributor to the low number of identified cross-links. We also demonstrate that sample preparation, digestion and peptide recovery are not causing biased losses against crosslinks. The low detection rates come mostly from severe crosslink signal splitting, as residues are participating in complex networks of reactive sites. |
| HostingRepository | PRIDE |
| AnnounceDate | 2025-11-19 |
| AnnouncementXML | Submission_2025-11-18_22:37:50.908.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Bruno Amaral |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Orbitrap Ascend |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-10-15 23:26:35 | ID requested | |
| ⏵ 1 | 2025-11-18 22:37:51 | announced | |
Publication List
Keyword List
| submitter keyword: Cross-linking, XL-MS, Reaction yield |
Contact List
| David C. Schiemer |
|---|
| contact affiliation | Department of Biochemistry and Molecular Biology, University of Calgary, Calgary, AB, Canada |
| contact email | dschriem@ucalgary.ca |
| lab head | |
| Bruno Amaral |
|---|
| contact affiliation | University of Calgary |
| contact email | bruno.amaral@ucalgary.ca |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/11/PXD069512 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD069512
- Label: PRIDE project
- Name: Signal Attrition in Whole Cell Crosslinking Mass Spectrometry
