⮝ Full datasets listing
PXD069490-1
PXD069490 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | YSB-phosphoproteomics cryptococcus using high resolution mass spectrometry, Yadav and Heitman |
| Description | Quantitative LC/MS/MS was performed using an EvoSep One UPLC coupled to a Thermo Orbitrap Astral high resolution accurate mass tandem mass spectrometer (Thermo). Briefly, each sample loaded EvoTip was eluted onto a 1.5 um EvoSep 150um ID x 15cm performance (EvoSep) column using the SPD30 gradient at 55C. Data collection on the Orbitrap Astral mass spectrometer was performed in a data-independent acquisition (DIA) mode of acquisition with a r=240,000 (m/z 200) full MS scan from m/z 380-1080 in the OT with a target AGC value of 4e5 ions. Fixed DIA windows of 5 m/z from m/z 380 to 1080 DIA MS/MS scans were acquired in the Astral with a target AGC value of 5e4 and max fill time of 8 ms. HCD collision energy setting of 27% was used for all MS2 scans. The total analysis cycle time for each sample injection was approximately 40 min |
| HostingRepository | MassIVE |
| AnnounceDate | 2025-12-01 |
| AnnouncementXML | Submission_2025-12-01_12:13:21.134.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Erik Soderblom |
| SpeciesList | scientific name: Cryptococcus |
| ModificationList | Phospho |
| Instrument | Orbitrap Astral |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2025-10-15 10:39:05 | ID requested | |
| ⏵ 1 | 2025-12-01 12:13:21 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: label free, astral, phosphorylation, DatasetType:Proteomics |
Contact List
| Joseph Heitman, MD PhD | |
|---|---|
| contact affiliation | Duke University |
| contact email | heitm001@duke.edu |
| lab head | |
| Erik Soderblom | |
| contact affiliation | Duke University |
| contact email | es114@duke.edu |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v11/MSV000099501/ |
