PXD069482 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Noncanonical amino acid-aided synthesis of anti-PD-L1 bispecific nanobody for colon cancer immunotherapy |
| Description | Colorectal cancer (CRC) is the second leading cause of cancer-related mortality, and the incidence of early-onset colon cancer has been increasing globally in recent years. The development of immunotherapies for colon cancer is critical for providing new treatment strategies to combat drug resistance. Here, a bispecific nanobody against PD-L1 (BsNb-PD-L1) constructed using genetically encoded noncanonical amino acids (ncAAs) is reported. A computational protocol was developed to identify appropriate sites in the nanobody for incorporating p-acetylphenylalanine (pAcF). Variants of nanobodies PV2 and PV3 with pAcF incorporated were conjugated with linkers containing an aminooxy functionality to enable oxime ligation. The resulting PV2-S71+PV3-N77 bispecific nanobody (BsNb-ncAA) exhibited higher thermostability and binding affinity compared to the nanobody monomers and the BsNb constructed by simply fusing two proteins. Moreover, in an in vitro phagocytosis model, the BsNb-ncAA exhibited improved capability to inhibit immune evasion and showed stronger biological activity compared to the fusion protein PV2-PV3. Furthermore, the BsNb-ncAA resulted in a marked increase in the number of CD8+ T cells within tumor tissues and demonstrated efficient inhibitory effects against colon tumor growth in vivo. Our study provides a general strategy for constructing BsNbs, which has potential applications in other cancer immunotherapy. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-01-12 |
| AnnouncementXML | Submission_2026-01-11_16:06:50.291.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Haoran Yu |
| SpeciesList | scientific name: Escherichia coli; NCBI TaxID: NEWT:562; |
| ModificationList | amidated residue |
| Instrument | autoflex TOF/TOF |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-10-15 06:12:06 | ID requested | |
| ⏵ 1 | 2026-01-11 16:06:51 | announced | |
Publication List
| 10.1038/s42003-025-09222-1; |
| Hu X, Jiang L, Yuan H, Chen W, Guo Y, Gao M, Song Y, Wang Z, Wang Y, Zhang M, de Marco A, Huang H, Kang G, Yu H, Noncanonical amino acid-aided synthesis of anti-PD-L1 bispecific nanobody for colon cancer immunotherapy. Commun Biol, 8(1):1844(2025) [pubmed] |
Keyword List
| submitter keyword: Bispecific antibody, maldi-tof,Noncanonical amino acid |
Contact List
| Haoran Yu |
|---|
| contact affiliation | Institute of Bioengineering, College of Chemical and Biological Engineering, Zhejiang University, Hangzhou, Zhejiang, China, 310027 |
| contact email | yuhaoran@zju.edu.cn |
| lab head | |
| Haoran Yu |
|---|
| contact affiliation | Zhejiang University |
| contact email | yuhaoran@zju.edu.cn |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD069482
- Label: PRIDE project
- Name: Noncanonical amino acid-aided synthesis of anti-PD-L1 bispecific nanobody for colon cancer immunotherapy
