PXD069473-1

PXD069473 is an original dataset announced via ProteomeXchange.

Title	Long-term exposure to biodegradable polylactic acid microplastics promotes Microcystis aeruginosa proliferation
Description	Biodegradable plastics, such as polylactic acid (PLA), are widely considered as sustainable alternatives to fossil fuel-derived polymers; however, their degradation generates polylactic acid microplastics (PLA-MPs), which raises ecological concerns in freshwater ecosystems. In this study, we investigated the responses of Microcystis aeruginosa to PLA-MPs (1, 10, and 50 mg/L) across a 45-day co-culture experiment. We found the PLA-MPs initially inhibited algal growth (days 18, 27) through oxidative stress, as indicated by elevated MDA content (1.94 nmol/mg protein at 50 mg/L, 1.57 times higher than the control) and SOD activity (1.30 U/mg Protein, 2.45 times higher than the control). However, during stabilization (days 36 and 45), PLA-MPs promoted growth in a dose-dependent manner, with Chl-a content in the 1, 10, and 50 mg/L groups reaching 1.12, 1.36, and 1.44 times that of the control, respectively. Concurrent shifts in inorganic carbon speciation, EPS secretion, and cellular aggregation highlighted dynamic adaptation strategies. Finally, based on the proteomic analysis, it was found that PLA-MPs promoted the growth of M. aeruginosa by improving photosynthetic efficiency, enhancing the expression of the TCA cycle and oxidative phosphorylation, and promoting cell division and proliferation. Our results confirm that PLA-MPs have a long-term effect on the growth of M. aeruginosa, providing fundamental theoretical guidance for investigating the ecological and environmental impacts of PLA-MPs in aquatic ecosystems.
HostingRepository	iProX
AnnounceDate	2025-10-15
AnnouncementXML	Submission_2025-10-15_00:40:25.894.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yao Qian
SpeciesList	scientific name: Microcystis aeruginosa; NCBI TaxID: 1126;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Astral

Revision	Datetime	Status	ChangeLog Entry
0	2025-10-15 00:40:04	ID requested
⏵ 1	2025-10-15 00:40:26	announced

Dataset with its publication pending

submitter keyword: Microcystis Aeruginosa, biodegraded microplastics, life history, influence mechanisms

Hong Li
contact affiliation	Key Laboratory of Eco-Environment of Three Gorges Region, Ministry of Education, Chongqing University
contact email	hongli@cqu.edu.cn
lab head
Yao Qian
contact affiliation	Key Laboratory of Eco-Environment of Three Gorges Region, Ministry of Education, Chongqing University
contact email	202517109505@stu.cqu.edu.cn
dataset submitter

iProX dataset URI