PXD069455-1

PXD069455 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	HDX-MS analysis of NKp46 with next gen antibodies
Description	Eptiopes of 7 unique antibodies produced by two complementary in vivo platforms utilizing a common light chain, in a chicken (OmniClic) or rat (OmniFlic) host species on NKp46 were analysed using HDX-MS. NKp46 was used as a model target antigen due to its use in emerging Natural Killer (NK) immune engagers that are being explored clinically as potentially safer alternatives to traditional CD3-based T cell engagers. We were able to successfully identify epitopes and map conformational changes of these antibodies validating our eptiope binning results from SPR.
HostingRepository	PRIDE
AnnounceDate	2025-11-27
AnnouncementXML	Submission_2025-11-27_04:49:56.322.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	John Burke
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 120

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2025-10-14 15:08:07	ID requested
⏵ 1	2025-11-27 04:49:57	announced

Publication List

10.1016/j.jbc.2025.110788;
Drew EE, Nyvall HG, Parson MAH, Talus RK, Burke JE, Hansen SD, SH2-mediated steric occlusion of the C2 domain regulates autoinhibition of SHIP1 inositol 5-phosphatase. J Biol Chem, 301(12):110788(2025) [pubmed]

10.1016/j.jbc.2025.110788;

Drew EE, Nyvall HG, Parson MAH, Talus RK, Burke JE, Hansen SD, SH2-mediated steric occlusion of the C2 domain regulates autoinhibition of SHIP1 inositol 5-phosphatase. J Biol Chem, 301(12):110788(2025) [pubmed]

Keyword List

submitter keyword: epitope, Antibody, NKp46,HDX

submitter keyword: epitope, Antibody, NKp46,HDX

Contact List

John E Burke
contact affiliation	University of Victoria
contact email	jeburke@uvic.ca
lab head
John Burke
contact affiliation	University of Victoria
contact email	jeburke@uvic.ca
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/11/PXD069455
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/11/PXD069455

PRIDE project URI

Repository Record List

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