PXD069416-1

PXD069416 is an original dataset announced via ProteomeXchange.

Title	Modular Assembly of Arterial Organoids Enables Rapid Blood Perfusion to Ameliorate Ischemic Diseases
Description	Transplantation of vascular cells remains a promising therapeutic strategy for enhancing vascular regeneration in the treatment of ischemic diseases. However, its clinical translation is hindered by poor engraftment and limited reparative potential. Here, we established a scalable methodology for generating suspensible, homogeneous arterial organoids (AOs) from human iPSCs using a three-dimensional bioreactor system. These spheroidal AOs constituted micron-scale vascular modules, enveloped by a confluent layer of arterial-like endothelial cells interfaced with mural cells. Local injection prompted the assembly of the modules into a larger tube, where the interstitial spaces between them constituted interconnected channels that supported rapid perfusion. To evaluate the therapeutic potential of this approach, we applied AOs in murine models of hindlimb ischemia and myocardial infarction. Upon delivery, the AOs rapidly anastomosed with the host vasculature, enhancing perfusion recovery and preserving tissue function, thereby outperforming single-cell counterparts. Mechanistically, the implanted AOs further recruited VEGFA-secreting macrophages to remodel the microenvironment, further reinforcing vascular regeneration. Collectively, our findings demonstrate that AOs can self-organize into perfusable vascular networks in vivo to restore blood flow in ischemic tissues. This study establishes injectable AOs as a promising therapeutic platform for the treatment of ischemic diseases.
HostingRepository	iProX
AnnounceDate	2025-10-14
AnnouncementXML	Submission_2026-08-11_00:06:55.687.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Zihang Pan
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 240

Revision	Datetime	Status	ChangeLog Entry
0	2025-10-13 20:25:41	ID requested
⏵ 1	2026-08-11 00:06:56	announced

Dataset with its publication pending

submitter keyword: iPSC, arterial organoids, self-organization, critical limb ischemia, ischemic heart disease, therapeutic vascularization.

Kai Wang
contact affiliation	Peking University
contact email	kai.wang88@pku.edu.cn
lab head
Zihang Pan
contact affiliation	Peking University
contact email	hanzpan@pku.edu.cn
dataset submitter

iProX dataset URI