PXD069398 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Interactomics of SARS-CoV-2 Macrodomain 1 Reveals Putative Clients of ADP-ribosyl Hydrolase Activity |
| Description | Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has greatly impacted public health due to high rates of transmissibility and mutation during the COVID-19 pandemic. Macrodomain 1 (Mac1) of non-structural protein 3 remained well-conserved across variants and is critical for suppression of host immune response to infection, making Mac1 a promising target for therapeutic development. Mac1 binds and cleaves the post-translational modification ADP-ribose and is hypothesized to have a downstream effect on host interferon response, but the exact cellular targets of Mac1 are still unknown. Characterizing the substrates of Mac1 ADP-ribosyl hydrolase activity using a catalytically inactive mutant N40D can reveal critical virus-host interactions to identify protein targets of Mac1 and reveal mechanisms of host interferon suppression. Here, we co-immunoprecipitated WT Mac1 and Mac1 N40D from HEK293T and A549 cell lines and quantified changes in protein interactions by TMT-multiplexed tandem mass spectrometry. We identified interactions between Mac1 and ADP-ribosylated substrates involved in DNA damage response, cytoskeletal components, and cell cycle regulation. Additionally, several members of the TRiC complex involved in protein folding were selectively enriched with mutant Mac1 from A549 cells. These findings suggest a novel role of Mac1 in regulating host protein folding. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-05-22 |
| AnnouncementXML | Submission_2026-05-22_07:58:56.860.xml |
| DigitalObjectIdentifier | https://doi.org/10.6019/PXD069398 |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Crissey Cameron |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | TMT6plex-126 reporter+balance reagent acylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
| Instrument | Orbitrap Exploris 480 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-10-13 08:01:25 | ID requested | |
| ⏵ 1 | 2026-05-22 07:58:57 | announced | |
Publication List
Keyword List
| submitter keyword: ADP-ribose, Macrodomain 1, affinity purification-mass spectrometry, Virus-host protein interactions, SARS-CoV-2, Interactomics |
Contact List
| Lars Plate |
|---|
| contact affiliation | Departments of Chemistry, Biological Sciences, Vanderbilt University and Pathology, Microbiology, and Immunology, Vanderbilt University Medical Center |
| contact email | lars.plate@vanderbilt.edu |
| lab head | |
| Crissey Cameron |
|---|
| contact affiliation | Vanderbilt University |
| contact email | crissey.d.cameron@vanderbilt.edu |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD069398
- Label: PRIDE project
- Name: Interactomics of SARS-CoV-2 Macrodomain 1 Reveals Putative Clients of ADP-ribosyl Hydrolase Activity
