PXD069395-1

PXD069395 is an original dataset announced via ProteomeXchange.

Title	Coculture of Collinsella aerofaciens and Bacteroides thetaiotaomicron under bile acid stress reveals vitamin B6 exchange
Description	Background: Although the bile acid-mediated microbiome-host interaction is known to shape both the composition and functionality of the gut microbiome, the mechanisms by which bile acid stress influences specific microbial metabolic interactions remain poorly understood. To address this gap, we examine the metabolic interplay between two key gut microbes. Bacteroides thetaiotaomicron, one of the most abundant species, possesses a broad enzymatic repertoire for polysaccharide degradation, while Collinsella aerofaciens is associated with liver-related diseases and plays a role in modifying primary bile acids. Results: In anaerobic coculture, C. aerofaciens mitigated the inhibitory effects of deoxycholic acid (DCA) on B. thetaiotaomicron by absorbing DCA from the medium and partially converting it into glycine-conjugated derivatives. Proteomic analysis showed that DCA broadly disrupted amino acid and vitamin metabolism pathways, particularly in B. thetaiotaomicron. In contrast, coculture led to a general upregulation of these pathways in C. aerofaciens, with a marked activation of vitamin B6 metabolism. Additionally, C. aerofaciens exhibited increased production of citrulline and ornithine in coculture. Conclusions: C. aerofaciens alleviates DCA toxicity on B. thetaiotaomicron through absorption, while promoting amino acid and vitamin metabolism, including the vitamin B6 synthesis pathway, during coculture. These results suggest that microbial interactions can enhance resistance to bile acid stress and may influence gut microbiome resilience, with potential relevance for liver- and bile acid–related disorders.
HostingRepository	PRIDE
AnnounceDate	2026-01-06
AnnouncementXML	Submission_2026-01-06_11:51:25.829.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD069395
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Yan Wang
SpeciesList	scientific name: Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482); NCBI TaxID: NEWT:226186; scientific name: Collinsella aerofaciens ATCC 25986; NCBI TaxID: NEWT:411903;
ModificationList	acetylated residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2025-10-13 06:38:45	ID requested
⏵ 1	2026-01-06 11:51:26	announced

10.6019/PXD069395;

submitter keyword: branched-chain amino acid, vitamin B6, Collinsella aerofaciens,gut-liver axis, deoxycholic acid, Bacteroides thetaiotaomicron, cross-feeding, bile acids, pyridoxal 5’-phosphate, arginine metabolism

Nico Jehmlich
contact affiliation	Helmholtz Centre for Environmental Research GmbH - UFZ;Department of Molecular Toxicology (MOLTOX); Group leader
contact email	nico.jehmlich@ufz.de
lab head
Yan Wang
contact affiliation	Helmholtz Centre for Environmental Research GmbH - UFZ; Department of Molecular Toxicology (MOLTOX)
contact email	yan.wang@ufz.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD069395
     1. Label: PRIDE project
     2. Name: Coculture of Collinsella aerofaciens and Bacteroides thetaiotaomicron under bile acid stress reveals vitamin B6 exchange