PXD069382 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | The RAB27A effector SYTL5 regulates mitophagy and mitochondrial metabolism |
| Description | SYTL5 is a member of the Synaptotagmin-Like (SYTL) protein family that differs from the Synaptotagmin family by having a unique N-terminal Synaptotagmin homology domain that directly interacts with the small GTPase RAB27A. Several SYTL protein family members have been implicated in plasma membrane transport and exocytosis, but the specific function of SYTL5 remains unknown. We here show that SYTL5 is a RAB27A effector and that both proteins localise to mitochondria and vesicles containing mitochondrial material. Mitochondrial recruitment of SYTL5 depends on its interaction with functional RAB27A. We demonstrate that SYTL5-RAB27A positive vesicles containing mitochondrial material, autophagy proteins and LAMP1 form during hypoxia and that depletion of SYTL5 and RAB27A reduces mitophagy under hypoxia mimicking conditions, indicating a role for these proteins in mitophagy. Indeed, we find that SYTL5 interacts with proteins involved in vesicle-mediated transport and cellular response to stress and that its depletion compromises mitochondrial respiration and increases glucose uptake. Intriguingly, SYTL5 expression is significantly reduced in tumours of the adrenal gland, and correlates positively with survival for patients with adrenocortical carcinoma. |
| HostingRepository | PRIDE |
| AnnounceDate | 2025-11-03 |
| AnnouncementXML | Submission_2025-11-02_23:33:05.474.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | SACHIN SINGH |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | timsTOF fleX |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-10-13 01:08:47 | ID requested | |
| ⏵ 1 | 2025-11-02 23:33:06 | announced | |
Publication List
Keyword List
| submitter keyword: SYTL5,RAB27A , mitochondrial metabolism, MS |
Contact List
| Tuula Anneli Nyman |
|---|
| contact affiliation | Proteomics Core Facility, Institute of Immunology, Rikshospitalet Oslo Norway |
| contact email | tuula.nyman@medisin.uio.no |
| lab head | |
| SACHIN SINGH |
|---|
| contact affiliation | Proteomics core facility, University Hospital, Oslo, Norway |
| contact email | sachin.singh@medisin.uio.no |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD069382
- Label: PRIDE project
- Name: The RAB27A effector SYTL5 regulates mitophagy and mitochondrial metabolism
