PXD069353 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | The dsRNA-proximal proteome during SARS-CoV-2 infection |
| Description | Coronaviruses establish replication organelles to synthesize their RNAs while evading host defenses. To systematically profile the host factors and the dynamic interplay between viral and host factors during viral replication, we develop an antibody-based proximity labeling strategy targeting double-stranded RNA (dsRNA), a hallmark of coronavirus replication intermediates, to map the proximal proteome at viral replication sites. Applying this to SARS-CoV-2–infected Calu-3 and HCoV-OC43–infected HCT-8, we reveal both conserved requirements and unique adaptations in each virus. Profiling during the early stage of SARS-CoV-2 infection further uncovers dynamic recruitment patterns: an early enrichment of ER proteins, mitochondrial proteins and RNA processing factors, and a progressive exclusion of stress granule components. Functional analyses of RNA-binding proteins identify regulatory host factors, including the double-stranded RNA-binding protein STAU1, which plays a pro-viral role on SARS-CoV-2 at the early infection stage. Collectively, these findings establish a robust proteomic framework for investigating coronavirus replication biology and uncover virus-specific strategies to manipulate host cellular machinery. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-07-27 |
| AnnouncementXML | Submission_2026-07-27_02:15:34.721.xml |
| DigitalObjectIdentifier | https://doi.org/10.6019/PXD069353 |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Sunghan Lee |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | biotinylated residue |
| Instrument | Orbitrap Eclipse |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-10-11 07:40:53 | ID requested | |
| ⏵ 1 | 2026-07-27 02:15:35 | announced | |
Publication List
Keyword List
| submitter keyword: Replication organelle,SARS-CoV-2, Proximity labeling |
Contact List
| V. Narry Kim |
|---|
| contact affiliation | Center for RNA Research, Institute for Basic Science, Seoul 08826, Korea School of Biological Sciences, Seoul National University, Seoul 08826, Korea |
| contact email | narrykim@snu.ac.kr |
| lab head | |
| Sunghan Lee |
|---|
| contact affiliation | Seoul National University |
| contact email | hanbio999@gmail.com |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/07/PXD069353 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD069353
- Label: PRIDE project
- Name: The dsRNA-proximal proteome during SARS-CoV-2 infection
