PXD069334-1

PXD069334 is an original dataset announced via ProteomeXchange.

Title	Bioactivity And Proteomic Analyses of Half-sandwich Iridium(III) Complexes
Description	Half-sandwich iridium(III) metallocomplexes represent a prospective structural type for the development of anticancer compounds. Our metallocomplexes showed high cytotoxicity in different cancer cell lines (2D and 3D cultures), including cisplatin-resistant lung carcinoma cells, while they were inactive against non-cancerous cells. For the first time, the effect of anticancer half-sandwich Ir complexes on the expression of stress-related genes associated with DNA damage and endoplasmic reticulum stress is discussed. Proteomic analysis reveals that 3 induces nucleolar stress and suppresses oxidative phosphorylation in A549 cells. This activates p53 and locks the RB/E2F axis, which is critical for initiating DNA replication. The cells are trapped before and during the S phase. The resulting cell phenotype is therefore predominantly cytostatic with apoptotic priming rather than purely genotoxic, as observed with cisplatin. The anti-cancer potential of selected metallocomplexe was proved in vivo where it affected tumor growth.
HostingRepository	PRIDE
AnnounceDate	2026-09-07
AnnouncementXML	Submission_2026-09-06_17:34:29.369.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	René Lenobel
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Bruker Daltonics timsTOF series

Revision	Datetime	Status	ChangeLog Entry
0	2025-10-10 14:19:04	ID requested
⏵ 1	2026-09-06 17:34:30	announced

10.1021/acs.jmedchem.5c03448;

Š, tarha P, Friedeck, á J, H, é, ž, ov, á R, B, á, rta O, Jaro, š, ov, á R, Ma, š, ek J, Nemec I, Milde D, Novobilsk, ý A, Novotn, ý L, Ondru, š J, Š, terbinsk, á S, Strakov, á N, Lenobel R, Ho, š, ek J, Mechanistic Exploration of Half-Sandwich Iridium(III) Anticancer Compounds through Integrated Cellular, Proteomic, and In Vivo Analyses. J Med Chem, 69(13):15166-15190(2026) [pubmed]

submitter keyword: human cells, LC-MSMS, proteomics,metallocomplexes, bioactivity

Rene Lenobel
contact affiliation	Laboratory of Growth Regulators Palacky University in Olomouc, Czech Republic
contact email	rene.lenobel@upol.cz
lab head
René Lenobel
contact affiliation	Palacky University in Olomouc, Department of protein biochemistry and proteomics
contact email	rene.lenobel@upol.cz
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/09/PXD069334

PRIDE project URI

• PRIDE
  1. PXD069334
     1. Label: PRIDE project
     2. Name: Bioactivity And Proteomic Analyses of Half-sandwich Iridium(III) Complexes