PXD069329 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Ribosomal architecture and rRNA modification landscape in the tick-borne parasite Babesia divergens |
| Description | Babesia are tick-borne intracellular apicomplexan parasites that infect a wide range of wild and domestic animals (e.g., cattle), resulting in significant economic losses to the livestock industry. Humans are considered accidental hosts for a few Babesia species. Babesia microti and B. divergens are the most prevalent causes of human babesiosis that are showing a broadening geographic distribution. Due to the complex life cycle of Babesia species, their survival depends on the precise control of gene expression, which is primarily regulated by epigenetic, transcriptional, and post-transcriptional mechanisms. High-resolution structural information on key components of the translation machinery, such as ribosomes, could aid in the development of antiparasitic drugs. Here, we report cryogenic electron microscopy ribosome structures from Babesia divergens, showing associated tRNAs, an mRNA fragment, and RACK1, a signaling scaffold crucial to translation regulation. Density map analysis displays ribosome regions at high resolution, which, when combined with nanopore sequencing, enabled the comprehensive identification of rRNA modifications, including modifications unreported in other organisms. The rRNA modifications localize not only to the reduced B. divergens rRNA expansion segments but also to functionally essential ribosomal sites. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-06-12 |
| AnnouncementXML | Submission_2026-06-12_11:07:55.304.xml |
| DigitalObjectIdentifier | https://doi.org/10.6019/PXD069329 |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Cristina Gutierrez-Vargas |
| SpeciesList | scientific name: Babesia divergens; NCBI TaxID: NEWT:32595; |
| ModificationList | acetylated residue; monohydroxylated residue; deaminated residue; iodoacetamide derivatized residue |
| Instrument | Orbitrap Fusion Lumos |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-10-10 11:35:28 | ID requested | |
| ⏵ 1 | 2026-06-12 11:07:55 | announced | |
| 2 | 2026-07-14 07:19:17 | announced | 2026-07-14: Updated project metadata. |
Publication List
Keyword List
| submitter keyword: ribosome, babesia, apicomplexan, translation |
Contact List
| Melissa Leger-Abraham |
|---|
| contact affiliation | Department of Microbiology, Blavatnik Institute, Harvard Medical School, Boston, MA, 02115, USA Division of Molecular Medicine, Boston Children's Hospital, Boston, MA, 02115, USA |
| contact email | melissa_leger-abraham@hms.harvard.edu |
| lab head | |
| Cristina Gutierrez-Vargas |
|---|
| contact affiliation | Department of Microbiology, Blavatnik Institute, Harvard Medical School, Boston, MA, 02115, USA |
| contact email | cristina_gutierrez-vargas@hms.harvard.edu |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD069329
- Label: PRIDE project
- Name: Ribosomal architecture and rRNA modification landscape in the tick-borne parasite Babesia divergens
