⮝ Full datasets listing
PXD069320-1
PXD069320 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Proteoform-Resolved Phosphorylation Dynamics in Kinase Complexes by Hybrid Precision Mass Spectrometry |
| Description | Our hybrid precision MS strategy integrates three complementary MS approaches to comprehensively characterize phosphorylation dynamics. (i) Intact protein reverse phase liquid chromatography mass spectrometry (RPLC-MS) quantifies proteoform changes over time , (ii) bottom-up MS resolves site-specific phosphorylation kinetics, and (iii) top-down fragmentation using ultra high-resolution MS definitively identifies proteoform architectures through sequencing. This integrated approach addresses individual method limitations by combining intact mass, sequence information, and temporal dynamics in a single workflow . |
| HostingRepository | MassIVE |
| AnnounceDate | 2026-04-08 |
| AnnouncementXML | Submission_2026-04-08_05:37:57.496.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Boris Krichel |
| SpeciesList | scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; |
| ModificationList | Phospho |
| Instrument | solariX; timsTOF Pro; impact II |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2025-10-10 08:00:36 | ID requested | |
| ⏵ 1 | 2026-04-08 05:37:58 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: AMPK, phosphorylation, proteoforms, top-down proteomics, bottom-up proteomics, mass spectrometry, kinase, post-translational modifications, hybrid mass spectrometry, phosphorylation dynamics, DatasetType:Proteomics |
Contact List
| Ying Ge | |
|---|---|
| contact affiliation | University of Wisconsin-Madison |
| contact email | ge2@wisc.edu |
| lab head | |
| Boris Krichel | |
| contact affiliation | University of Luebeck |
| contact email | bkrichel@gmail.com |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v11/MSV000099446/ |
