PXD069286-1
PXD069286 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | A novel tool for labeling intermediary proteins between two non-interacting proteins |
| Description | This dataset contains the raw data of mass spectrometry from a STUPPIT pull-down experiment investigating the intermediary proteins between two non-interacting proteins. We developed STUPPIT (Split-TurboID and PUP-IT based Protein Identification Tool), a novel method to biotinylate intermediary proteins of two non-interacting proteins through a two-step enzymatic reaction. STUPPIT were validated using proteomics targeting YAP1&β-actin (Subproject IPX0013559002, using STUPPIT all-in-plasmid method, 3 repeats), YAP1&β-actin and YAP1&MOB1A (IPX0013559004,using stably cell line STUPPIT approach I, 2 repeats ) as shown in each subgroup. New intermediary proteins enriched by STUPPIT between β-catenin and SMAD4, key components of the Wnt and BMP signaling pathways, were also subjected to proteomics (Subproject IPX0013559003, 3 repeats). In conclusion, STUPPIT represents a powerful tool for labeling and capturing intermediary proteins between non-interacting partners, offering new insights into protein-protein interactions and advancing signal transduction research. |
| HostingRepository | iProX |
| AnnounceDate | 2025-10-09 |
| AnnouncementXML | Submission_2025-10-10_00:49:13.637.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Wenxiu Ning |
| SpeciesList | scientific name: Homo sapiens; NCBI TaxID: 9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Orbitrap Exploris 480; Orbitrap Fusion Lumos |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2025-10-10 00:48:47 | ID requested | |
| ⏵ 1 | 2025-10-10 00:49:14 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: STUPPIT, proximity labeling, non-interacting proteins, intermediary proteins, proteomics |
Contact List
| Wenxiu Ning | |
|---|---|
| contact affiliation | Yunnan university |
| contact email | wenxiu_ning@ynu.edu.cn |
| lab head | |
| Wenxiu Ning | |
| contact affiliation | Yunnan university |
| contact email | wenxiu_ning@ynu.edu.cn |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
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