PXD069280-1

PXD069280 is an original dataset announced via ProteomeXchange.

Title	Distinct Necrotic Protein Cleavages as Biomarkers for Necrosis
Description	Apoptosis is executed by caspase-mediated proteolytically cleavage. In contrast, we know very little regarding the involvement of proteolytic cleavage in necrosis. In particular, we still lack mechanistic insights and biomarkers for necrosis caused by overwhelming stress such as ferroptosis. Here we found that necrosis, such as necroptosis and ferroptosis, led to characteristic necrotic lamin-B1 cleavage distinct from that of apoptotic lamin-B1 cleavage, while both apoptotic and necrotic cleavages of lamin-B1 were found in pyroptosis. Using neo-N-terminomic analysis, we identified a substantial set of protein substrates proteolytically cleaved after Arg/Lys residues in necrosis mediated by extracellular trypsin-like proteases. Necrotic protein cleavage is involved in mediating nuclear morphological change and loss of chromatin DNA in necrosis and the clearance of necrotic dead cells by phagocytosis. Defective necrotic protein cleavage promotes autoimmunity. Finally, we developed antibodies recognizing specific necrotic cleavage events as biomarkers for necrosis in cells and in vivo under pathological conditions.
HostingRepository	iProX
AnnounceDate	2025-10-09
AnnouncementXML	Submission_2026-03-15_19:58:24.785.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Bing Shan
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606; scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationList	dimethylated residue
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2025-10-10 00:29:58	ID requested
⏵ 1	2026-03-15 19:58:25	announced

Dataset with its publication pending

submitter keyword: Protein Cleavage Biomarkers of Necrosis

Yaoyang Zhang
contact affiliation	Shanghai institute of Organic Chemistry,CAS
contact email	zyy@sioc.ac.cn
lab head
Bing Shan
contact affiliation	Shanghai institute of Organic Chemistry,CAS
contact email	shanbing@sioc.ac.cn
dataset submitter

iProX dataset URI