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PXD069279-1

PXD069279 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Proximity labeling-based proteomic analysis of the HEV ORF1-associated replication complex under EIF4H deficiency
Description	This project explores the role of EIF4H in regulating the composition of the HEV ORF1-associated replication complex using proximity-dependent biotin labeling with TurboID. TurboID was inserted into a validated permissive site within the ORF1 coding region that allows insertion without disrupting ORF1 function. HEK293T wild-type and EIF4H-knockout cells expressing ORF1-TurboID were subjected to biotin labeling, streptavidin-based enrichment, and mass spectrometry analysis. Comparative proteomics identified EIF4H-dependent host factors associated with the HEV replication complex.
HostingRepository	iProX
AnnounceDate	2025-10-09
AnnouncementXML	Submission_2025-10-10_00:28:48.330.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Dong Lin
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Ascend

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2025-10-10 00:28:19	ID requested
⏵ 1	2025-10-10 00:28:48	announced

Publication List

Dataset with its publication pending

Keyword List

submitter keyword: HEV, ORF1, proximity labeling

Contact List

Qiang Ding
contact affiliation	Tsinghua University
contact email	qding@tsinghua.edu.cn
lab head
Dong Lin
contact affiliation	Tsinghua University
contact email	dong-l19@mails.tsinghua.edu.cn
dataset submitter

Full Dataset Link List

iProX dataset URI