PXD069261 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Development of a chemoproteomic platform to identify the sites of (homo)citrullination within complex proteomes |
| Description | Citrullination and homocitrullination of arginine and lysine can significantly impact protein structure and function. Citrullination of arginine is an enzymatic modification catalyzed by the Protein Arginine Deiminases (PADs). Homocitrullination of lysine is a non-enzymatic modification that occurs in the presence of high concentrations of cyanate. Both post-translational modifications are elevated in Rheumatoid Arthritis (RA) and other inflammatory diseases. Moreover, autoantibodies targeting these PTMs are associated with the development of RA. Identifying arginine and lysine residues that are hypersensitive to these modifications is critical for deepening our understanding of the functional effects of (homo)citrullination. Current methods use phenylglyoxal-biotin for the protein-level identification of (homo)citrullinated proteins, however, this platform does not inform on the exact site of citrullination. Herein we describe the development of a desthiobiotin-phenylglyoxal (DB-PG) probe, which can be used to selectively enrich and subsequently release (homo)citrullinated peptides for the site-specific identification of (homo)citrullinated arginines and lysines. Coupled with TMT quantification, this method monitors citrullination in ~800 arginine residues and ranks ~1400 lysine residues by homocitrullination sensitivity. Projecting forward, this platform will enable the comprehensive analysis of (homo)citrullination in complex proteomes. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-03-10 |
| AnnouncementXML | Submission_2026-03-09_20:05:25.252.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Sarah Mann |
| SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090; |
| ModificationList | N6-carbamoyl-L-lysine; L-citrulline |
| Instrument | Orbitrap Exploris 240 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-10-09 12:43:43 | ID requested | |
| ⏵ 1 | 2026-03-09 20:05:25 | announced | |
Publication List
Keyword List
| submitter keyword: phenylglyoxal, chemoproteomics, desthiobiotin,Citrulline, homocitrulline |
Contact List
| Eranthie Weerapana |
|---|
| contact affiliation | Boston College |
| contact email | eranthie@bc.edu |
| lab head | |
| Sarah Mann |
|---|
| contact affiliation | Boston College |
| contact email | mannsc@bc.edu |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/03/PXD069261 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD069261
- Label: PRIDE project
- Name: Development of a chemoproteomic platform to identify the sites of (homo)citrullination within complex proteomes
