PXD069219-1

PXD069219 is an original dataset announced via ProteomeXchange.

Title	Mapping the placental galectin-3 interactome identifies CD9 and ITGB1 as functional glycoprotein counter-receptors during syncytialization.
Description	The fetomaternal interface is replete with glycan-binding proteins (GBPs) that can interact with cell surface glycoprotein counter-receptors to regulate placental function. Here, we interrogate the role of galectin-3, a GBP that controls placental trophoblast syncytialization, an important differentiation process where progenitor cytotrophoblast cells fuse to produce the multinucleated syncytiotrophoblast. The molecular mechanism of galectin-3-mediated fusion has not yet been elucidated in part due to the difficulty of studying glycan-GBP binding events in live cells. To overcome these challenges, we employ a proximity labeling strategy to identify the galectin-3 interactome. From this interactome dataset, we selected and validated CD9 and integrin beta 1 as functional counter-receptors of galectin-3 and showed that CD9 is glycosylated with an N-linked glycan at a rare non-canonical sequon. Furthermore, we present evidence that galectin-3 acts to physically alter the fluidity of the cellular membrane and it does not activate canonical syncytialization signaling pathways. Overall, we report that galectin-mediated binding events and their corresponding functions in cell biology can be precisely regulated by select glycoproteins at specific glycosites.
HostingRepository	PRIDE
AnnounceDate	2025-11-24
AnnouncementXML	Submission_2025-11-23_16:09:41.777.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD069219
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Abigail Reeves
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2025-10-08 16:25:58	ID requested
⏵ 1	2025-11-23 16:09:42	announced

10.6019/PXD069219;

10.1073/pnas.2511042122;

Reeves AE, Yang GS, Baboo S, Diedrich JK, Bedekar P, Farhadi SA, Wanchoo A, Bratcher C, Hudalla GA, Yates JR, Huang ML, Mapping the placental galectin-3 interactome identifies CD9 and ITGB1 as functional glycoprotein counterreceptors during syncytialization. Proc Natl Acad Sci U S A, 122(45):e2511042122(2025) [pubmed]

submitter keyword: glycosylation, trophoblast, proximity labeling, tetraspanin, mass spectrometry (MS)-based proteomics, syncytialization,galectin-3, glycan-binding protein

Mia L Huang
contact affiliation	Professor Dept of Chemistry Scripps Research Institute
contact email	miahuang@scripps.edu
lab head
Abigail Reeves
contact affiliation	The Scripps Research Institute
contact email	areeves@scripps.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD069219
     1. Label: PRIDE project
     2. Name: Mapping the placental galectin-3 interactome identifies CD9 and ITGB1 as functional glycoprotein counter-receptors during syncytialization.