PXD069185-1

PXD069185 is an original dataset announced via ProteomeXchange.

Title	UFMylation orchestrates chromatin engagement of core NHEJ components to promote DNA double-strand break repair
Description	DNA double-strand breaks (DSBs) are highly cytotoxic lesions that – if misrepaired – can lead to genomic instability, cancer, and developmental disorders. While ubiquitin and select ubiquitin-like proteins (UBLs) are established regulators of DSB repair, many UBLs remain understudied. Here, through systematic UBL screening, we identify UFM1 as a novel regulator of non-homologous end-joining (NHEJ). Using a photo-crosslinkable UFM1 probe, we uncover core NHEJ components, including XRCC4, as downstream UFM1 receptors. Complementary proximity screening reveals UFMylated Ku70 as a key upstream target recognised by XRCC4. Mechanistically, UFMylation facilitates chromatin recruitment and assembly of NHEJ factors, establishing a central role in end-joining repair. Our findings link hereditary UFMylation disorders to defective NHEJ processes, contributing to our molecular understanding of diseases biology. Beyond this, our bottom-up screening strategy reveals broader networks of UFM1-regulated factors, featuring diverse roles in genome maintenance and cellular homeostasis.
HostingRepository	PRIDE
AnnounceDate	2026-07-31
AnnouncementXML	Submission_2026-07-31_08:25:42.790.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Petra Beli
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2025-10-07 04:34:55	ID requested
⏵ 1	2026-07-31 08:25:43	announced

10.1038/s41467-026-73882-8;

Wang Z, Foster BM, da Costa IC, Wu Y, Behera D, Conte F, Trotter EW, Lopezcolorado FW, Cabello-Lobato MJ, Choudhary S, Wiener R, Beli P, Smith DL, Banks WH, Bagley S, McKee S, Minnis M, Meyer S, Chaplin AK, D, ö, rner W, Mootz HD, Hagan IM, Galanty Y, Stark JM, Larrosa I, Cliff MJ, Schmidt CK, Systematic discovery of UFM1 receptors reveals a regulatory module in DNA repair directing non-homologous end-joining. Nat Commun, 17(1):(2026) [pubmed]

submitter keyword: double-strand break, proximity proteomics,UFMylation, NHEJ

Petra Beli
contact affiliation	Institute of Molecular Biology gGmbH(IMB), Mainz, 55128, Germany Institute for Developmental Biology and Neurobiology (iDN) University of Mainz, Germany
contact email	p.beli@imb-mainz.de
lab head
Petra Beli
contact affiliation	Institute of Molecular Biology (IMB), Mainz, Germany
contact email	p.beli@imb-mainz.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/07/PXD069185

PRIDE project URI

• PRIDE
  1. PXD069185
     1. Label: PRIDE project
     2. Name: UFMylation orchestrates chromatin engagement of core NHEJ components to promote DNA double-strand break repair