PXD069183 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Affinity-purified interactants of reovirus muNS |
| Description | Mammalian orthoreovirus (reovirus) nonstructural protein μNS nucleates viral factories (VFs), which are sites of viral genome replication, protein synthesis, and particle assembly. Reovirus mRNAs are not polyadenylated, yet these transcripts are efficiently translated. To identify host factors associated with translation in VFs, we conducted a proteomics screen and identified ataxin-2-like (ATXN2L) as a μNS-interacting protein. CRISPR-mediated gene knockout (KO) of ATXN2L impairs reovirus replication. The ATXN2L RNA-binding domains are required for reovirus replication and association with the 3’ terminus of nonpolyadenylated reovirus mRNAs. Synthesis of viral proteins is diminished in ATXN2L-KO cells following reovirus infection. Translation of a reovirus reporter construct is diminished following transfection of ATXN2L-KO cells with nonpolyadenylated mRNA but not with polyadenylated mRNA. These data identify ATXN2L as an essential mediator of translation of nonpolyadenylated reovirus mRNA. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-01-12 |
| AnnouncementXML | Submission_2026-01-12_03:46:59.011.xml |
| DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD069183 |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Terence Dermody |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; scientific name: Mammalian orthoreovirus 3 Dearing; NCBI TaxID: NEWT:10886; |
| ModificationList | biotinylated residue |
| Instrument | LTQ Orbitrap Velos |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-10-07 03:15:04 | ID requested | |
| 1 | 2025-11-24 14:23:31 | announced | |
| ⏵ 2 | 2026-01-12 03:47:00 | announced | 2026-01-12: Updated project metadata. |
Publication List
| 10.1038/s41467-025-67547-1; |
| Somoulay X, Treadaway GS, Hedayati S, Steed ME, Adam NE, Williams MS, Parker JSL, Woolford JL, Silva LA, Taylor GM, Dermody TS, Ataxin-2-like promotes translation of nonpolyadenylated reovirus mRNA. Nat Commun, 17(1):842(2025) [pubmed] |
| 10.6019/PXD069183; |
Keyword List
Contact List
| Terence Shawn |
|---|
| contact affiliation | Department of Pediatrics University of Pittsburgh USA |
| contact email | terence.dermody@chp.edu |
| lab head | |
| Terence Dermody |
|---|
| contact affiliation | University of Pittsburgh |
| contact email | dms231@pitt.edu |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/11/PXD069183 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD069183
- Label: PRIDE project
- Name: Affinity-purified interactants of reovirus muNS
