PXD069182-1

PXD069182 is an original dataset announced via ProteomeXchange.

Title	Characterization of Androctonus mauretanicus Venom and In Vitro Screening of SARS-CoV-2 Entry Inhibitors Candidates
Description	The project focuses on the characterization and exploration of the bioactive components of Androctonus mauritanicus scorpion venom, with an emphasis on proteomic profiling and peptide identification. The overarching goal is to generate a comprehensive dataset of venom peptides and proteins, which can serve as a resource for understanding venom composition, discovering novel bioactive molecules, and facilitating comparative analyses with other scorpion species. Specimens were collected from a high-risk region for scorpion envenomation and maintained under controlled laboratory conditions. Venom was extracted via low-voltage electrical stimulation, centrifuged to remove impurities, lyophilized, and stored at −80°C to preserve biological activity. The venom was then solubilized, desalted, and fractionated using solid-phase extraction and reverse-phase high-performance liquid chromatography, allowing for separation of complex protein mixtures into manageable fractions suitable for mass spectrometry analysis. Enzymatic digestion was performed using trypsin and Lys-C, following reduction and alkylation steps to break disulfide bonds and prevent their reformation. Peptide samples were concentrated and analyzed using high-resolution mass spectrometry, including both Quadrupole Time-of-Flight (Q-TOF) and Orbitrap Q-Exactive platforms, coupled with nano-flow liquid chromatography. Data-dependent acquisition strategies were employed to maximize peptide coverage, and advanced software tools were used to process raw spectra, identify peptide sequences, and annotate post-translational modifications. The resulting dataset provides high-confidence peptide identifications, including sequence information suitable for deposition in public proteomics repositories. By sharing these data through ProteomeXchange, the project aims to enhance reproducibility, enable comparative studies, and support further research into venom-derived bioactive compounds with potential therapeutic applications.
HostingRepository	PRIDE
AnnounceDate	2025-10-08
AnnouncementXML	Submission_2025-10-08_01:06:32.560.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Reda Chahir
SpeciesList	scientific name: mixed culture metagenome; NCBI TaxID: NEWT:1306859;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF; 6220 Time-of-Flight LC/MS; Q Exactive; 6410 Triple Quadrupole LC/MS; LTQ Orbitrap; 6520A Quadrupole Time-of-Flight LC/MS

Revision	Datetime	Status	ChangeLog Entry
0	2025-10-07 01:11:57	ID requested
⏵ 1	2025-10-08 01:06:33	announced

10.3389/FPHAR.2025.1678606;

submitter keyword: proteomics, antiviral peptides, mass spectrometry, SARS-CoV-2, scorpion venom,Androctonus mauritanicus

Naouale Oukkache
contact affiliation	Venoms and Toxins Laboratory, Institut Pasteur of Morocco, Casablanca
contact email	naoual.oukkache@gmail.com
lab head
Reda Chahir
contact affiliation	Laboratory of Venoms and Toxins, Pasteur Institute of Morocco, 1 Place Louis Pasteur, Casablanca 20250, Morocco. Laboratory of Agri-food and Health, Faculty of Sciences and Techniques Settat (FSTS) , Hassan I University (UH1) , Settat Km 3, P.O. Box 577, Casablanca Road
contact email	r.chahif@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/10/PXD069182

PRIDE project URI

• PRIDE
  1. PXD069182
     1. Label: PRIDE project
     2. Name: Characterization of Androctonus mauretanicus Venom and In Vitro Screening of SARS-CoV-2 Entry Inhibitors Candidates