PXD069167-1

PXD069167 is an original dataset announced via ProteomeXchange.

Title	m⁶A-Dependent Interaction Between circSMAD2 and IGF2BP2 Upregulates SMAD2 and Drives Nasopharyngeal Carcinoma Progression
Description	Nasopharyngeal carcinoma (NPC) is a malignant tumor of the head and neck, with tumor recurrence and distant metastasis being major contributors to poor patient prognosis. However, the underlying molecular mechanisms remain incompletely understood. Circular RNAs (circRNAs) have emerged as important regulators in NPC progression. In this study, we identified circSMAD2 as significantly upregulated in NPC tissues, with high expression correlating with poor clinical outcomes. Functional assays in vitro and in vivo demonstrated that circSMAD2 promotes NPC cell invasion and lung colonizationmetastasis. Mechanistically, circSMAD2 binds to IGF2BP2 and facilitates its nuclear export in an m⁶A-dependent manner, facilitating IGF2BP2’s recognition of m⁶A modification sites on SMAD2 mRNA, thereby increasing SMAD2 mRNA stability and expression. The elevated SMAD2 subsequently activates key epithelial-mesenchymal transition (EMT) regulators ZEB1 and TWIST, promoting NPC cell invasiveness and metastatic potential. This study identifies a previously uncharacterized role of circSMAD2 in NPC and suggests that it may regulate the parental gene SMAD2 via m⁶A modification. These results contribute to understanding NPC biology and may inform future therapeutic strategies.
HostingRepository	PRIDE
AnnounceDate	2025-10-10
AnnouncementXML	Submission_2025-10-09_20:21:49.477.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	佳蓉 张
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2025-10-06 08:53:42	ID requested
⏵ 1	2025-10-09 20:21:50	announced

Dataset with its publication pending

submitter keyword: Nasopharyngeal carcinoma

circSMAD2

IGF2BP2

SMAD2

Invasion and metastasis

Jiarong, Zhang
contact affiliation	No. 88 Xiangya Road Street, Changsha City/District, Hunan Province, central south of university
contact email	2117057675@qq.com
lab head
佳蓉 张
contact affiliation	central south of university
contact email	2117057675@qq.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD069167
     1. Label: PRIDE project
     2. Name: m⁶A-Dependent Interaction Between circSMAD2 and IGF2BP2 Upregulates SMAD2 and Drives Nasopharyngeal Carcinoma Progression