PXD069136 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Systematic discovery of UFM1 receptors reveals a regulatory module in DNA repair directing non-homologous end-joining |
| Description | As the most recently identified UBL, UFM1 is garnering increasing attention for its roles beyond its canonical functions at the endoplasmic reticulum. To address the challenge of defining UFM1 substrate-receptor relationships, we develop a fully functional, photo-crosslinkable UFM1 probe for receptor screening. The approach identifies a broad spectrum of UFM1-interacting proteins, spanning biological processes known to be regulated by UFM1 as well as unknown associations. Notably, several core NHEJ components ranked prominently amongst the candidates. Using additional biochemical and biophysical techniques, including crosslinking mass spectrometry (CX-MS), we validate and characterise non-canonical UFM1-binding regions on several NHEJ proteins, including XRCC4. In addition, using recombinant reagents, we have identified potential UFMylation sites of Ku70 and Ku80 through identification of VG-K remnant peptides following trypsin digestion. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-06-25 |
| AnnouncementXML | Submission_2026-06-25_03:09:21.086.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Benjamin Foster |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | acetylated residue; monohydroxylated residue; deamidated residue |
| Instrument | Orbitrap Fusion Lumos |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-10-05 04:20:01 | ID requested | |
| ⏵ 1 | 2026-06-25 03:09:21 | announced | |
Publication List
Keyword List
| submitter keyword: UFM1 |
| ubiquitin-like proteins (UBLs) |
| DNA repair |
| DNA double-strand breaks (DSBs) |
| non-homologous end-joining (NHEJ) |
| XRCC4 |
| genetic code expansion |
| photo-crosslinking |
| proximity labelling |
Contact List
| Christine Schmidt |
|---|
| contact affiliation | Manchester Cancer Research Centre, Division of Cancer Sciences, School of Medical Sciences, Faculty of Biology, Medicine and Health, University of Manchester, Manchester, UK |
| contact email | christine.schmidt@manchester.ac.uk |
| lab head | |
| Benjamin Foster |
|---|
| contact affiliation | Manchester Cancer Research Centre, Division of Cancer Sciences, Faculty of Biology, Medicine and Health, University of Manchester |
| contact email | benjamin.foster@manchester.ac.uk |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD069136 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD069136
- Label: PRIDE project
- Name: Systematic discovery of UFM1 receptors reveals a regulatory module in DNA repair directing non-homologous end-joining
