PXD069135-1

PXD069135 is an original dataset announced via ProteomeXchange.

Title	Proteomic profiling of tendon-specific VHL knockout and VHL-dependent double knockout mouse tail tendon fascicles
Description	This study investigates how chronic activation of HIF1α in tendon cells drives pathological extracellular matrix (ECM) remodeling and whether these changes can be modulated by genetic rescue strategies. Using tendon cell-specific deletion of Vhl (Scx;Vhl) in mice, which stabilizes HIF1α, we observed proteomic signatures consistent with maladaptive matrix remodeling, including upregulation of wound-associated proteins and loss of structural collagens. To disentangle the contribution of HIF1α from HIF2α, we generated double knockout mice lacking both Vhl and Hif1a in tendon cells (Scx;Vhl;Hif1α), which normalized many of the ECM changes and mechanical defects, demonstrating a central role of HIF1α. In parallel, to test whether vascular infiltration contributes to matrix pathology, we crossed Scx;Vhl mice with Vegfa floxed mice (Scx;Vhl;Vegfa). While VEGFA deletion prevented neovascularization and reduced innervation, ECM proteomics and mechanical properties largely remained pathological, indicating that HIF1α-dependent ECM remodeling occurs independently of vascular ingrowth. Together, this dataset provides a comprehensive proteomic characterization of tendon ECM changes induced by HIF1α stabilization and clarifies the distinct roles of HIF1α and VEGFA-driven angiogenesis in tendon disease.
HostingRepository	PRIDE
AnnounceDate	2025-11-24
AnnouncementXML	Submission_2025-11-24_03:31:16.398.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Greta Moschini
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2025-10-05 03:17:10	ID requested
⏵ 1	2025-11-24 03:31:16	announced

Dataset with its publication pending

submitter keyword: LC-MSMS, VEGFA KO,Mouse, HIF1a KO, Tendon, VHL KO

Jess Gerrit Snedeker
contact affiliation	Laboratory of Orthopedic Biomechanics, ETH Zurich (Department of Health Sciences and Technology) and University of Zurich (Medical Faculty)
contact email	jess.snedeker@hest.ethz.ch
lab head
Greta Moschini
contact affiliation	ETH Zürich
contact email	gretam@ethz.ch
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/11/PXD069135

PRIDE project URI

• PRIDE
  1. PXD069135
     1. Label: PRIDE project
     2. Name: Proteomic profiling of tendon-specific VHL knockout and VHL-dependent double knockout mouse tail tendon fascicles