PXD069119 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | HigH-ratiO partiaL proteolysiS with carriER proteome (HOLSER) Enables Global Structure Profiling and Site-resolved Elucidation of Ligand-Protein Interactions |
| Description | Understanding how cellular proteins interact with their environment, including endogenous and exogeneous molecules, is critical for elucidating mechanisms of cellular regulation and drug action. Partial proteolysis-based techniques offer peptide-level resolution of ligand-induced conformational changes but are limited by modest proteome coverage and depth, as well as sensitivity to the experimental conditions. To overcome these limitations, we developed higH ratiO partiaL proteolysiS with carriER proteome (HOLSER), an efficient workflow that features extended digestion time for low peptide yield variability and tandem mass tag (TMT)-based multiplexing that includes full digests for enhanced proteome depth and sequence coverage as well as higher precision of abundance measurements. We demonstrate HOLSER capabilities across diverse usage cases, including global proteome structure profiling, kinase target mapping, and structural mapping of the FKBP-mTOR complex in response to rapamycin. HOLSER reveals domain-specific stabilization and identifies peptides in direct contact with ligands, providing a powerful and scalable platform for simultaneous probing structural changes at the scale of the whole proteome, individual protein domains and specific binding sites. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-06-08 |
| AnnouncementXML | Submission_2026-06-07_18:43:40.452.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Massimiliano Gaetani |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue |
| Instrument | Orbitrap Exploris 480 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-10-03 06:35:34 | ID requested | |
| ⏵ 1 | 2026-06-07 18:43:41 | announced | |
Publication List
| Lyu H, Gharibi H, Meng Z, Sokolova B, Lundstr, รถ, m S, Zhang X, Zubarev RA, Peptide-to-Protein Data Aggregation Using Fisher's Method Improves Target Identification in Chemical Proteomics. Anal Chem, 98(17):12596-12602(2026) [pubmed] |
| 10.1021/acs.analchem.5c08021; |
Keyword List
| submitter keyword: proteomics, TMT,Drug Traget deconvolution, LC-MS, machanism of action, partial proteolysis |
Contact List
| Massimiliano Gaetani |
|---|
| contact affiliation | Chemical Proteomics, Division of Chemistry I, Department of Medical Biochemistry and Biophysics Karolinska Institutet, Stockholm, Sweden |
| contact email | massimiliano.gaetani@ki.se |
| lab head | |
| Massimiliano Gaetani |
|---|
| contact affiliation | Karolinska Institutet |
| contact email | massimiliano.gaetani@ki.se |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD069119 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD069119
- Label: PRIDE project
- Name: HigH-ratiO partiaL proteolysiS with carriER proteome (HOLSER) Enables Global Structure Profiling and Site-resolved Elucidation of Ligand-Protein Interactions
