PXD069099-1

PXD069099 is an original dataset announced via ProteomeXchange.

Title	The glycosaminoglycan-dependent interactome of neurexin-1 in human fetal glial cells
Description	Neurexins are synaptic adhesion molecules best characterized in neurons, where they regulate synapse assembly and function, with emerging evidence indicating they are also abundantly expressed by astrocytes. To elucidate the interactome of NRXN1α, we employed a proximity labeling strategy in cultured human fetal glial cells (SVG p12 cells). This approach enables the identification of transient and spatially restricted protein interactions, offering insights into the molecular environment of NRXN1α in glia. Further, we investigated how the presence and number of glycosaminoglycan (GAG) chains present on NRXN1α influence these interactions by generating glycosylation-deficient mutants at previously characterized GAG glycosites. Here, we show that the astrocytic NRXN1α interactome in SVG p12 cells consists of over 400 proteins, half of which are likely modulated by GAGs. Our findings provide a systems-level view of NRXN1α-associated proteins in fetal glia cultured in the absence of neurons and highlight the role of GAG valency in modulating its interactome.
HostingRepository	PRIDE
AnnounceDate	2025-12-22
AnnouncementXML	Submission_2025-12-21_16:10:52.443.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD069099
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Mia Huang
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2025-10-02 15:24:02	ID requested
⏵ 1	2025-12-21 16:10:53	announced

10.6019/PXD069099;

10.1002/pgr2.70039;

Critcher M, Wu H, Lu Y, Huang ML, The Glycosaminoglycan-Dependent Interactome of Neurexin-1 in Human Fetal Glial Cells. Proteoglycan Res, 3(4):(2025) [pubmed]

submitter keyword: proximity tagging, glycosaminoglycan,Interactome, thrombospondin-1, neurexin-1

Mia L. Huang
contact affiliation	Professor Dept of Chemistry Scripps Research Institute
contact email	miahuang@scripps.edu
lab head
Mia Huang
contact affiliation	Scripps Research Institute
contact email	glycotimelab@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD069099
     1. Label: PRIDE project
     2. Name: The glycosaminoglycan-dependent interactome of neurexin-1 in human fetal glial cells