PXD069054 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | A Simple Human Embryo Model Reveals V-ATPase Requirement in Mammalian Blastocyst Cavitation |
| Description | Human naïve pluripotent stem cells (nPSCs) can differentiate into trophectoderm (TE) and self-organize into embryo-like structures known as blastoids, typically requiring complex cocktails of signaling factors. Here, we demonstrate that dimethyl sulfoxide (DMSO) alone robustly induces TE differentiation and blastoid formation, recapitulating peri-implantation development, including amniotic cavity formation. Using this simplified system, we uncover that lysosomal pathways—particularly those involving the proton pump V-ATPase—are essential for blastoid cavitation. DMSO upregulates key V-ATPase subunits (ATP6V0A4 and ATP6V1B1), which are also upregulated in human embryo TE. Inhibition of V-ATPase in the DMSO model disrupted lysosomal acidification, blocked intracellular vacuole formation, and impaired blastoid cavitation. Furthermore, genetic and pharmacological disruption of V-ATPase function significantly impaired cavitation in mouse and human blastocysts. Thus, our simple DMSO model effectively recapitulates human blastocyst cavitation and reveals an essential, conserved mechanism involving V-ATPase-mediated lysosomal acidification during early embryogenesis |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-03-12 |
| AnnouncementXML | Submission_2026-03-12_04:36:31.268.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Huoming Zhang |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | timsTOF Pro 2 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-10-01 15:28:21 | ID requested | |
| ⏵ 1 | 2026-03-12 04:36:31 | announced | |
Publication List
Keyword List
| submitter keyword: Blastoids |
| trophectoderm differentiation |
| dimethyl sulfoxide |
| V-ATPase |
| lysosomal acidification |
Contact List
| Huoming Zhang |
|---|
| contact affiliation | Analytical Chemistry Core Lab (ACL) – Proteomics Unit Building 2 east, Level 2, 2281-WS05 King Abdullah University of Science and Technology (KAUST) Thuwal, 23955-6900, Kingdom of Saudi Arabia |
| contact email | huoming.zhang@kaust.edu.sa |
| lab head | |
| Huoming Zhang |
|---|
| contact affiliation | King Abdullah University of Science and Technology |
| contact email | huoming.zhang@kaust.edu.sa |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/03/PXD069054 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD069054
- Label: PRIDE project
- Name: A Simple Human Embryo Model Reveals V-ATPase Requirement in Mammalian Blastocyst Cavitation
