⮝ Full datasets listing
PXD069052-1
PXD069052 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Diverse HIV Env immunogens with similar germline-targeting modifications drive distinct B cell receptor changes targeting the same epitope |
| Description | VRC01-class HIV broadly neutralizing antibodies target the receptor-binding site on the HIV Env protein and have been isolated from people living with HIV. Their predicted unmutated (germline) forms do not display detectable binding to Env and do not neutralize HIV. They acquire their broadly neutralizing potentials through the accumulation of specific somatic mutations. Here we identify key modifications that allow Env-derived proteins from diverse HIV clades to effectively engage germline VRC01-class B cell receptors (BCRs) expressed by naive B cells. Noticeably, the way these germline-targeting Env proteins interact with VRC01-class BCRs varied depending on the specific Env background. When used as immunogens, all tested germline-targeting Envs successfully activate VRC01-class B cells in vivo. However, the resulting BCRs accumulate distinct mutations at key positions. Thus, while the same BCRs are initially activated, they follow different maturation pathways depending on the immunogen used. These findings have important implications not only for HIV vaccine design efforts, but also for immunogens aiming to direct specific BCR maturation pathways in other contexts. |
| HostingRepository | MassIVE |
| AnnounceDate | 2026-04-01 |
| AnnouncementXML | Submission_2026-04-01_22:14:05.902.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Sabyasachi Baboo |
| SpeciesList | scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; scientific name: Human immunodeficiency virus 1; NCBI TaxID: 11676; |
| ModificationList | Oxidation; Deamidated:18O(1); HexNAc; Gln->pyro-Glu |
| Instrument | Orbitrap Eclipse; Q Exactive HF-X |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2025-10-01 15:02:42 | ID requested | |
| ⏵ 1 | 2026-04-01 22:14:06 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: N-glycosylation, DeGlyPHER, VRC01, bNAb, HIV Env, Vaccine, DatasetType:Proteomics |
Contact List
| John R.Yates III | |
|---|---|
| contact affiliation | The Scripps Research Institute |
| contact email | jyates@scripps.edu |
| lab head | |
| Sabyasachi Baboo | |
| contact affiliation | The Scripps Research Institute, La Jolla, CA |
| contact email | sbaboo@scripps.edu |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v11/MSV000099354/ |
