PXD069040-1

PXD069040 is an original dataset announced via ProteomeXchange.

Title	Infrared light enhances deep-sea yeast growth through temperature insensitive wavelength-dependent mitochondrial metabolism
Description	Deep-sea hydrothermal vents emit intense infrared (IR) radiation that profoundly shapes microbial ecology and promotes bacterial growth. To investigate the mechanisms underlying IR responsiveness, we isolated Rhodotorula mucilaginosa strain F-J1, a deep-sea yeast. We found that both elevated temperature and near-infrared (NIR, 810–1050 nm) irradiation stimulated F-J1 growth. Whereas dark-grown cultures were highly temperature-sensitive, NIR irradiation—particularly at 880 nm—not only enhanced growth but also substantially lowered the Arrhenius activation energy, indicating a wavelength-dependent, temperature-insensitive stimulation mechanism. At the atomic level, substituting hydrogen with deuterium (via heavy water) suppressed F-J1 growth. This inhibition was partially alleviated by wavelength-dependent NIR irradiation, implicating water’s overtone vibrations. However, deuterium completely abolished the stimulatory effect of 880 nm NIR, revealing a distinct, wavelength-specific mechanism. Inhibition of mitochondrial ribosome function and oxidative phosphorylation impaired NIR-stimulated growth, underscoring the critical role of mitochondrial metabolism. Electron microscopy further showed NIR-induced mitochondrial morphological changes consistent with heightened metabolic activity. Proteomic analysis identified upregulation of mitochondrial ribosomal protein S18, suggesting enhanced mitochondrial protein biosynthesis under optimal NIR conditions. Together, these findings uncover a mitochondria-centered, non-thermal IR-sensing mechanism that parallels known photobiomodulation processes in mammalian cells. This work provides new insights into the ecological role of IR radiation in deep-sea hydrothermal ecosystems and highlights its potential biomedical and biotechnological applications.
HostingRepository	PRIDE
AnnounceDate	2026-09-07
AnnouncementXML	Submission_2026-09-06_16:17:21.251.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Salomé Sauvage
SpeciesList	scientific name: Rhodotorula mucilaginosa; NCBI TaxID: NEWT:5537;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2025-10-01 11:39:23	ID requested
⏵ 1	2026-09-06 16:17:22	announced

Dai J, Zhang L, Li Y, Zhao R, Li XG, Liu J, Kosta A, Le Guenno H, Sauvage S, Zhang WJ, Lebrun R, Shen Y, Yang H, Chen C, Wu LF, Infrared stimulation of mitochondrial metabolism in deep-sea yeast expands the energetic framework of hydrothermal ecosystems. ISME Commun, 6(1):ycag129(2026) [pubmed]

10.1093/ismeco/ycag129;

submitter keyword: blackbody emission

deep-sea hydrothermal vents

infrared

wavelength

photobiomodulation

Long-Fei WU
contact affiliation	Laboratoire de Chimie Bacterienne, UMR7283 CNRS-AMU, 31 Chemin Joseph Aiguier, 13009 Marseille, France
contact email	wu@imm.cnrs.fr
lab head
Salomé Sauvage
contact affiliation	Proteomics Facility of Institut de Microbiologie de la Méditerranée, CNRS, 31 Chemin Joseph Aiguier, 13009 Marseille
contact email	salome.sauvage@orange.fr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD069040
     1. Label: PRIDE project
     2. Name: Infrared light enhances deep-sea yeast growth through temperature insensitive wavelength-dependent mitochondrial metabolism