PXD069026-1

PXD069026 is an original dataset announced via ProteomeXchange.

Title	Ancestral sequence reconstruction reveals CTP-dependent parS binding as an ancient feature of ParB DNA segregation proteins
Description	In most bacteria, chromosome and low-copy plasmid segregation are mediated by the ParABS system. Its component ParB functions as a DNA sliding clamp that assembles on centromere-like parS sites to form large nucleoprotein complexes, which are subsequently positioned by the ATPase ParA. Loading of ParB onto DNA is regulated by a recently discovered, conserved CTPase domain, yet the evolutionary origin of this regulatory module remains unclear. Here, we apply ancestral sequence reconstruction to resurrect anci¬ent ParB proteins dating back to the last common ancestor of bacteria. Biochemical, structural, and cell biological analyses demonstrate that these reconstructed proteins display all core activities of their mod¬ern counterparts, suggesting that the ParABS system emerged early during bacterial evolution and has essentially re¬mained unchanged ever since. More broadly, our findings indicate that regulatory CTP¬ases repre¬sent an ancient molecular innovation, whose origins can be traced back to the earliest stages of life.
HostingRepository	PRIDE
AnnounceDate	2026-01-07
AnnouncementXML	Submission_2026-01-07_09:16:36.285.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Wieland Steinchen
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: NEWT:562;
ModificationList	No PTMs are included in the dataset
Instrument	Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2025-10-01 01:33:20	ID requested
⏵ 1	2026-01-07 09:16:36	announced

10.1016/J.JMB.2025.169611;

ProteomeXchange project tag: Hydrogen Deuterium Exchange (HDX-MS)

submitter keyword: CTP, chromosome segregation, evolution,Bacteria, ParABS system

Gert Bange
contact affiliation	1 Max Planck Fellow Group Molecular Physiology of Microbes, Max Planck Institute for Terrestrial Microbiology, Marburg, Germany 2 Center for Synthetic Microbiology (SYNMIKRO), Marburg, Germany 3 Department of Chemistry, University of Marburg, Marburg, Germany
contact email	gert.bange@synmikro.uni-marburg.de
lab head
Wieland Steinchen
contact affiliation	Philipps-University Marburg, Department of Chemistry & SYNMIKRO, Karl-von-Frisch-Straße 14, 35043 Marburg, GERMANY
contact email	wieland.steinchen@synmikro.uni-marburg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/01/PXD069026

PRIDE project URI

• PRIDE
  1. PXD069026
     1. Label: PRIDE project
     2. Name: Ancestral sequence reconstruction reveals CTP-dependent parS binding as an ancient feature of ParB DNA segregation proteins